The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions.

The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions. Research Abstract Details 

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The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions. Abstract Text:

Huili Zhang,Jianwei He,Yanyan Ji,Akio Kato,Youtao Song,

We cultured calnexin-disrupted and wild-type Saccharomyces cerevisiae strains under conditions of heat stress. The growth rate of the calnexin-disrupted yeast was almost the same as that of the wild-type yeast under those conditions. However, the induced mRNA level of the molecular chaperone PDI in the ER was clearly higher in calnexin-disrupted S. cerevisiae relative to the wild type at 37 degrees C, despite being almost the same in the two strains under normal conditions. The western blotting analysis for PDI protein expression in the ER yielded results that show a parallel in their mRNA levels in the two strains. We suggest that PDI may interact with calnexin under heat stress conditions, and that the induction of PDI in the ER can recover part of the function of calnexin in calnexin-disrupted yeast, and result in the same growth rate as in wild-type yeast.

The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions. Publishing Authors By Initials

H Zhang,J He,Y Ji,A Kato,Y Song,

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The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cellular & molecular biology letters

VOLUME: 13

Page Numbers: 38-48

Journal Abbreviation: Cell. Mol. Biol. Lett.

ISSN: 1425-8153

DAY: 19

MONTH: 10

YEAR: 2007

The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions. Information

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LANGUAGE: eng

NlmUniqueID: 9607427

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Grant and Affiliation Information for The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions.

AFFILIATION: Department of Life Science, Liaoning University, Shenyang, 110036, China, ysong@lnu.edu.cn.

Country: Poland

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MEDLINETA: Cell Mol Biol Lett

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