The effect of actin disrupting agents on contact guidance of human embryonic stem cells.

The effect of actin disrupting agents on contact guidance of human embryonic stem cells. Research Abstract Details 

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The effect of actin disrupting agents on contact guidance of human embryonic stem cells. Abstract Text:

Sharon Gerecht,Christopher J Bettinger,Zhitong Zhang,Jeffrey T Borenstein,Gordana Vunjak-Novakovic,Robert Langer,

Mammalian cells respond to their substrates by complex changes in gene expression profiles, morphology, proliferation and migration. We report that substrate nanotopography alters morpohology and proliferation of human embryonic stem cells (hESCs). Fibronectin-coated poly(di-methyl siloxane) substrates with line-grating (600nm ridges with 600nm spacing and 600+/-150nm feature height) induced hESC alignment and elongation, mediated the organization of cytoskeletal components including actin, vimentin, and alpha-tubulin, and reduced proliferation. Spatial polarization of gamma-tubulin complexes was also observed in response to nanotopography. Furthermore, the addition of actin disrupting agents attenuated the alignment and proliferative effects of nanotopography. These findings further demonstrate the importance of interplay between cytoskeleton and substrate interactions as a key modulator of morphological and proliferative cellular response in hESCs on nanotopography.

The effect of actin disrupting agents on contact guidance of human embryonic stem cells. Publishing Authors By Initials

S Gerecht,CJ Bettinger,Z Zhang,JT Borenstein,G Vunjak-Novakovic,R Langer,

For similar macromolecular substances: polymers: biopolymers: microtubule proteins: tubulin research abstracts see: macromolecular substances: polymers: biopolymers: microtubule proteins: tubulin research

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The effect of actin disrupting agents on contact guidance of human embryonic stem cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biomaterials

VOLUME: 28

Page Numbers: 4068-77

Journal Abbreviation: Biomaterials

ISSN: 0142-9612

DAY: 18

MONTH: 06

YEAR: 2007

The effect of actin disrupting agents on contact guidance of human embryonic stem cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8100316

The effect of actin disrupting agents on contact guidance of human embryonic stem cells. Keywords Mesh Terms:

KEYWORDS: Tubulin

MESH TERMS: metabolism

Chemical & Substance for Abstract: The effect of actin disrupting agents on contact guidance of human embryonic stem cells. Information

Substance Name: latrunculin B

Registry Number: 76343-94-7

Grant and Affiliation Information for The effect of actin disrupting agents on contact guidance of human embryonic stem cells.

AFFILIATION: Harvard-M.I.T. Division of Health Sciences and Technology, USA.

Country: England

AGENCY: United States NIDCR

GRANT: R01-DE-013023-06

ACRONYM: DE

MEDLINETA: Biomaterials

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