The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli.

The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli. Research Abstract Details 

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The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli. Abstract Text:

H Homma,T Kobayashi,N Chiba,K Karasawa,H Mizushima,I Kudo,K Inoue,H Ikeda,M Sekiguchi,S Nojima,

The nucleotide sequence of the pldA gene of Escherichia coli K-12, which codes for detergent-resistant phospholipase A (DR-phospholipase A), located in the outer membrane, was determined and the amino acid sequence of DR-phospholipase A was deduced. DR-phospholipase A contains 269 amino acids, resulting in a protein with a molecular weight of 30,809. It does not contain any cysteine residues and seems to be synthesized first as a precursor with a typical signal peptide composed of 20 amino acids. The NH2-terminus of the mature protein is glutamine, a polar amino acid, while other outer membrane proteins so far determined have a nonpolar amino acid there. The hydropathy profile of the deduced amino acid sequence of DR-phospholipase A was studied. Most of the region was rather hydrophilic and there were no stretches of hydrophobic amino acids. Computer analysis showed that there are no homologies between DR-phospholipase A and other extracellular phospholipases whose amino acid sequences are known. The candidates for the promoter region of the pldA gene, the 5'-flanking region, have a significantly high AT content, while the AT content of the coding region is about the same as the average AT content of the E. coli chromosome. A typical rho-independent transcription termination site is also present at the 3'-flanking region. This is the first example of the primary structure of a membrane-bound phospholipase.

The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli. Publishing Authors By Initials

H Homma,T Kobayashi,N Chiba,K Karasawa,H Mizushima,I Kudo,K Inoue,H Ikeda,M Sekiguchi,S Nojima,

For similar peptides: protein sorting signals research abstracts see: peptides: protein sorting signals research

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The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 96

Page Numbers: 1655-64

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1984

The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli. Information

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LANGUAGE: eng

NlmUniqueID: 376600

The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli. Keywords Mesh Terms:

KEYWORDS: Protein Sorting Signals

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli. Information

Substance Name: Phospholipases A

Registry Number: EC 3.1.1.-

Grant and Affiliation Information for The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli.

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Country: JAPAN

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MEDLINETA: J Biochem

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ACCESSION NUMBER: X02143

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