The differential contractile responses to capsaicin and anandamide in muscle strips isolated from the rat urinary bladder.

The differential contractile responses to capsaicin and anandamide in muscle strips isolated from the rat urinary bladder. Research Abstract Details 

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The differential contractile responses to capsaicin and anandamide in muscle strips isolated from the rat urinary bladder. Abstract Text:

Chikashi Saitoh,Chika Kitada,Wataru Uchida,Michael B Chancellor,William C de Groat,Naoki Yoshimura,

The contractile responses to capsaicin and anandamide, exogenous and endogenous agonists for transient receptor potential vanilloid receptor 1 (TRPV1), respectively, were investigated in muscle strips isolated from the rat urinary bladder. Capsaicin and anandamide produced concentration-dependent contractions of the muscle strips. The contractile response induced by capsaicin disappeared within approximately 20 min. In contrast, anandamide produced contractile responses lasting at least for 30 min. Capsaicin produced additive contractile responses in anandamide-treated muscle strips. The contractile response to anandamide was attenuated, but not abolished in strips desensitized by capsaicin. The response to capsaicin was abolished in the presence of a TRPV1 antagonist, N-(4-tertiarybutylphenyl)-4-(3-chlorphyridin-2-yl)tetrahydropyrazine-1(2H)-carbox-amide (BCTC), but not altered in the presence of either tetrodotoxin, atropine or indomethacin. In the presence of SR140333, a tachykinin NK(1) receptor antagonist or SR48968, an NK(2) receptor antagonist, the response to capsaicin was attenuated. The response to anandamide was partially attenuated in the presence of ONO8130, a prostanoid EP(1) receptor antagonist, URB597, a fatty-acid amide hydrolase inhibitor, BCTC, SR140333 or SR48968, and almost completely abolished by indomethacin. Neither tetrodotoxin, atropine, a cannabinoid CB(1) receptor antagonist, AM251, nor a cannabinoid CB(2) receptor antagonist, AM630, had any effect on the response to anandamide. These results indicate that capsaicin produces muscle contractions by stimulating the TRPV1 receptor, followed by release of neuropeptides that can activate tachykinin NK(1) and/or NK(2) receptors in the bladder and that the contractile response to anandamide is mediated at least in part by activation of prostanoid EP(1) receptors due to production of prostaglandins in addition to TRPV1 receptor activation.

The differential contractile responses to capsaicin and anandamide in muscle strips isolated from the rat urinary bladder. Publishing Authors By Initials

C Saitoh,C Kitada,W Uchida,MB Chancellor,WC de Groat,N Yoshimura,

For similar urogenital system: urinary tract: urinary bladder research abstracts see: urogenital system: urinary tract: urinary bladder research

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The differential contractile responses to capsaicin and anandamide in muscle strips isolated from the rat urinary bladder. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: European journal of pharmacology

VOLUME: 570

Page Numbers: 182-7

Journal Abbreviation: Eur. J. Pharmacol.

ISSN: 0014-2999

DAY: 5

MONTH: 06

YEAR: 2007

The differential contractile responses to capsaicin and anandamide in muscle strips isolated from the rat urinary bladder. Information

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LANGUAGE: eng

NlmUniqueID: 1254354

The differential contractile responses to capsaicin and anandamide in muscle strips isolated from the rat urinary bladder. Keywords Mesh Terms:

KEYWORDS: Urinary Bladder

MESH TERMS: physiology

Chemical & Substance for Abstract: The differential contractile responses to capsaicin and anandamide in muscle strips isolated from the rat urinary bladder. Information

Substance Name: anandamide

Registry Number: 94421-68-8

Grant and Affiliation Information for The differential contractile responses to capsaicin and anandamide in muscle strips isolated from the rat urinary bladder.

AFFILIATION: Pharmacology Laboratories, Astellas Pharma Inc., 21 Miyukigaoka, Tsukuba, Ibaraki 305-8585, Japan.

Country: Netherlands

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MEDLINETA: Eur J Pharmacol

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