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The different distribution of asialo GM1 and Forssman antigen in the small intestine of mouse demonstrated by immunofluorescence staining.

The different distribution of asialo GM1 and Forssman antigen in the small intestine of mouse demonstrated by immunofluorescence staining. Research Abstract Details 

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  • The different distribution of asialo GM1 and Forssman antigen in the small intestine of mouse demonstrated by immunofluorescence staining. Abstract Text:

    a suzukiA Suzuki,t yamakawaT Yamakawa,

    Our previous studies demonstrated clearly the presence of asialo GM1 in the intestinal mucosa and microvillus membranes of mouse. The present work demonstrated the distribution of asialo GM1 as well as the entirely complementary distribution of Forssman antigen in the small intestine of mouse by immunofluorescence staining. Clear staining of the brush border and basolateral membranes of epithelial cells, the cell membranes of cryptic cells and also some secretory granules was observed with the purified rabbit anti-asialo GM1 IgG and fluorescence-conjugated goat anti-rabbit IgG. On the other hand, Forssman antigen was demonstrated neither on the brush border membranes nor cryptic membranes, but demonstrated distinctly in the mesenchymal tissue. Such a remarkable difference of distribution of the two glycolipids, which are biosynthesized by different pathways from a common precursor glycolipid (lactosylceramide), indicates that the expression of sugar transferases for elongation of carbohydrate units may be regulated by precise genetic information during organ differentiation.

    The different distribution of asialo GM1 and Forssman antigen in the small intestine of mouse demonstrated by immunofluorescence staining. Publishing Authors By Initials

    a suzukiA Suzuki,t yamakawaT Yamakawa,

    For similar investigative techniques: clinical laboratory techniques: cytological techniques: histocytological preparation techniques: staining and labeling research abstracts see: investigative techniques: clinical laboratory techniques: cytological techniques: histocytological preparation techniques: staining and labeling research

    PUBMED ID PMID:

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    The different distribution of asialo GM1 and Forssman antigen in the small intestine of mouse demonstrated by immunofluorescence staining. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Journal of biochemistry

    VOLUME: 90

    Page Numbers: 1541-4

    Journal Abbreviation: J. Biochem.

    ISSN: 0021-924X

    DAY: 19

    MONTH: Nov

    YEAR: 1981

    The different distribution of asialo GM1 and Forssman antigen in the small intestine of mouse demonstrated by immunofluorescence staining. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 376600

    The different distribution of asialo GM1 and Forssman antigen in the small intestine of mouse demonstrated by immunofluorescence staining. Keywords Mesh Terms:

    KEYWORDS: Staining and Labeling

    MESH TERMS: immunology

    Chemical & Substance for Abstract: The different distribution of asialo GM1 and Forssman antigen in the small intestine of mouse demonstrated by immunofluorescence staining. Information

    Substance Name: Forssman Antigen

    Registry Number: 9013-60-9

    Grant and Affiliation Information for The different distribution of asialo GM1 and Forssman antigen in the small intestine of mouse demonstrated by immunofluorescence staining.

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    Country: JAPAN

    JAPAN Research PublicationJAPAN Research Publication

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    MEDLINETA: J Biochem

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