The development of alternative vitrification solutions for microencapsulated islets.

The development of alternative vitrification solutions for microencapsulated islets. Research Abstract Details 

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The development of alternative vitrification solutions for microencapsulated islets. Abstract Text:

Carlos A Agudelo,Hiroo Iwata,

Bioartificial pancreas in which islets of Langerhans (islets) are enclosed in a semipermeable membrane is one of the approaches to treat insulin-dependent diabetic patients. Although there are advantages in this method, one of the issues that still remains is the long-term storage of tissue engineering devices before transplantation. One of the possible routes to address this is through cryopreservation. In this study, a freezing solution, 2m DMSO in RPMI-1640, a conventional vitrification solution, VS55, and the newly developed vitrification solution KYO-1 were examined to cryopreserve microencapsulated islets in agarose hydrogel. The insulin release ability, morphology of islets, and physico-chemical properties of the agarose gel membrane were examined after a cryopreservation and thawing process. Frozen and vitrified (by KYO-1) groups showed a similar insulin secretion. Frozen groups by 2m DMSO, however, showed destruction of agarose capsules and some islets were out of the capsule. When KYO-1 was used, islets still maintained the ability to release insulin in response to glucose stimulation, and agarose capsule showed morphological integrity, and mechanical properties. In conclusion, vitrification using KYO-1 which is composed of 5.38m ethylene glycol, 2m DMSO, 0.1m PEG 1000 and 0.00175m PVP K10 in EuroCollins, is a suitable method for cryopreservation of microencapsulated islets.

The development of alternative vitrification solutions for microencapsulated islets. Publishing Authors By Initials

CA Agudelo,H Iwata,

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The development of alternative vitrification solutions for microencapsulated islets. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biomaterials

VOLUME: 29

Page Numbers: 1167-76

Journal Abbreviation: Biomaterials

ISSN: 0142-9612

DAY: 20

MONTH: 12

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 8100316

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Grant and Affiliation Information for The development of alternative vitrification solutions for microencapsulated islets.

AFFILIATION: Department of Regenerative Materials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.

Country: England

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MEDLINETA: Biomaterials

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