The detection of human sapoviruses with universal and genogroup-specific primers.

The detection of human sapoviruses with universal and genogroup-specific primers. Research Abstract Details 

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The detection of human sapoviruses with universal and genogroup-specific primers. Abstract Text:

M Okada,Y Yamashita,M Oseto,K Shinozaki,M Okada,Y Yamashita,M Oseto,K Shinozaki,M Okada,Y Yamashita,M Oseto,K Shinozaki,

Sapovirus (SV) causes gastroenteritis in humans and comprises genetically divergent viruses. A nested reverse transcription-polymerase chain reaction (RT-PCR) targeting the capsid-protein-coding region was developed using universal and genogroup-specific primer sets. The universal primers were capable of detecting human SV genogroups I, II, IV and V. Genetic analysis of the amplified products enabled us to phylogenetically determine the genotypes of the viruses. In addition, genogroup-specific primers that amplified different lengths of the amplicon depending on the genogroup were developed. These genogroup-specific primers were also used as inner primers for the nested PCR. These two simple RT-PCR methods are powerful tools for both detection and epidemiological studies of human SV.

The detection of human sapoviruses with universal and genogroup-specific primers. Publishing Authors By Initials

M Okada,Y Yamashita,M Oseto,K Shinozaki,M Okada,Y Yamashita,M Oseto,K Shinozaki,M Okada,Y Yamashita,M Oseto,K Shinozaki,

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The detection of human sapoviruses with universal and genogroup-specific primers. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Archives of virology

VOLUME: 151

Page Numbers: 2503-9

Journal Abbreviation: Arch. Virol.

ISSN: 0304-8608

DAY: 19

MONTH: 07

YEAR: 2006

The detection of human sapoviruses with universal and genogroup-specific primers. Information

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LANGUAGE: eng

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Grant and Affiliation Information for The detection of human sapoviruses with universal and genogroup-specific primers.

AFFILIATION: Division of Virology, Chiba Prefectural Institute of Public Health, Chiba, Japan.

Country: Austria

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MEDLINETA: Arch Virol

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