The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae.

The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae. Abstract Text:

The mechanisms by which environmental carbon sources regulate biofilm formation are poorly understood. This study investigates the roles of glucose and the catabolite repression system in Serratia marcescens biofilm formation. The abilities of this opportunistic pathogen to proliferate in a wide range of environments, to cause disease, and to resist antimicrobials are linked to its ability to form biofilms. We observed that growth of S. marcescens in glucose-rich medium strongly stimulated biofilm formation, which contrasts with previous studies showing that biofilm formation is inhibited by glucose in Escherichia coli and other enteric bacteria. Glucose uptake is known to inversely mediate intracellular cyclic AMP (cAMP) synthesis through regulation of adenylate cyclase (cyaA) activity, which in turn controls fundamental processes such as motility, carbon utilization and storage, pathogenesis, and cell division in many bacteria. Here, we demonstrate that mutation of catabolite repression genes that regulate cAMP levels (crr and cyaA) or the ability to respond to cAMP (crp) confers a large increase in biofilm formation. Suppressor analysis revealed that phenotypes of a cAMP receptor protein (crp) mutant require the fimABCD operon, which is responsible for type 1 fimbria production. Consistently, fimA transcription and fimbria production were determined to be upregulated in a cyaA mutant background by using quantitative real-time reverse transcription-PCR and transmission electron microscopy analysis. The regulatory pathway by which environmental carbon sources influence cAMP concentrations to alter production of type 1 fimbrial adhesins establishes a novel mechanism by which bacteria control biofilm development.

The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Applied and environmental microbiology

VOLUME: 74

Page Numbers: 3461-70

Journal Abbreviation: Appl. Environ. Microbiol.

ISSN: 1098-5336

DAY: 18

MONTH: 04

YEAR: 2008

The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7605801

The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae.

AFFILIATION: Charles T. Campbell Laboratory of Ophthalmic Microbiology, Department of Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA.

Country: United States

AGENCY: United States NEI

GRANT: EY08098

ACRONYM: EY

MEDLINETA: Appl Environ Microbiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae Related Publications

• A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards.
• Amitriptyline and imipramine poisoning in children.
• Base up prism spectacles as visual aid in patients treated for macular hole.
• Childhood epilepsy.
• Dexamethasone-induced effects on lymphocyte distribution and expression of adhesion molecules in treatment-resistant depression.
• Dual concerns with the dualist approach.
• End-of-life education.
• Gonococcal ophthalmia neonatorum despite treatment with antibacterial eye-drops.
• Hydrolysis of oligosaccharides from distillers grains using organic-inorganic hybrid mesoporous silica catalysts.
• Hypotension caused by L-dopa.
• Identification of chicken-specific fecal microbial sequences using a metagenomic approach.
• Identification of hexose hydrolysis products in metabolic flux analytes: A case study of levulinic acid in plant protein hydrolysate.
• Long-term results of macular pucker surgery.
• Meningococcaemia and the purpura of Martin de Gimard; report of a case.
• Metabolic flux maps comparing the effect of temperature on protein and oil biosynthesis in developing soybean cotyledons.
• Mode of action of verapamil.
• Models of covariation-based causal judgment: a review and synthesis.
• Paradoxical effects of base rates and representation in category learning.
• Pathology of renal cell carcinoma: recent developments.
• Platform biochemicals for a biorenewable chemical industry.
• Predictors of difficult intubation in patients with cervical spine limitations.
• Studies of glaucoma.
• THE COMPATIBILITY OF OCTAPRESSIN WITH CYCLOPROPANE, TRICHLORETHYLENE AND HALOTHANE.
• The anatomical bases of semantic retrieval deficits in early Alzheimer's disease.
• The changing incidence of convulsions in childhood.
• The cyclic AMP-dependent catabolite repression system of Serratia marcescens mediates biofilm formation through regulation of type 1 fimbriae.
• The historical background of convulsions in childhood.
• The separation of radiodiagnosis and radiotherapy in Great Britain: a symbiosis.
• The treatment of bone sarcoma.
• What Really Happened during the 1918 Influenza Pandemic? The Importance of Bacterial Secondary Infections.