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The complete amino acid sequence of rice bran trypsin inhibitor.

The complete amino acid sequence of rice bran trypsin inhibitor. Research Abstract Details 

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  • The complete amino acid sequence of rice bran trypsin inhibitor. Abstract Text:

    m tashiroM Tashiro,k hashinoK Hashino,m shiozakiM Shiozaki,f ibukiF Ibuki,z makiZ Maki,

    The complete amino acid sequence of a double-headed trypsin inhibitor (RBTI) from rice bran was determined by a combination of limited proteolysis of the native inhibitor with Streptomyces griseus trypsin at pH 3 and conventional methods. RBTI consists of 133 amino acid residues including 18 half-cystine residues which are involved in 9 disulfide bridges in the molecule. The limited proteolysis at pH 3 produced a major split of Lys(83)-Met(84) and a minor split of Arg(107)-Val(108) together with a non-enzymatic hydrolysis of Asp(19)-Pro(20) in the molecule. The established sequence showed that RBTI is composed of 4 domains, domains I and III, and domains II and IV being homologous to the first and the second domains of soybean Bowman-Birk inhibitor, respectively, indicating that RBTI has a duplicated structure of the Bowman-Birk type inhibitor.

    The complete amino acid sequence of rice bran trypsin inhibitor. Publishing Authors By Initials

    m tashiroM Tashiro,k hashinoK Hashino,m shiozakiM Shiozaki,f ibukiF Ibuki,z makiZ Maki,

    For similar chemical actions and uses: pharmacologic actions: molecular mechanisms of pharmacological action: enzyme inhibitors: protease inhibitors: serine proteinase inhibitors: trypsin inhibitors research abstracts see: chemical actions and uses: pharmacologic actions: molecular mechanisms of pharmacological action: enzyme inhibitors: protease inhibitors: serine proteinase inhibitors: trypsin inhibitors research

    PUBMED ID PMID:

    MEDLINE DATE:

    The complete amino acid sequence of rice bran trypsin inhibitor. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Journal of biochemistry

    VOLUME: 102

    Page Numbers: 297-306

    Journal Abbreviation: J. Biochem.

    ISSN: 0021-924X

    DAY: 19

    MONTH: Aug

    YEAR: 1987

    The complete amino acid sequence of rice bran trypsin inhibitor. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 376600

    The complete amino acid sequence of rice bran trypsin inhibitor. Keywords Mesh Terms:

    KEYWORDS: Trypsin Inhibitors

    MESH TERMS: analysis

    Chemical & Substance for Abstract: The complete amino acid sequence of rice bran trypsin inhibitor. Information

    Substance Name: Trypsin Inhibitors

    Registry Number: 0

    Grant and Affiliation Information for The complete amino acid sequence of rice bran trypsin inhibitor.

    AFFILIATION: Department of Food Science and Nutrition, Faculty of Living Science, Kyoto Prefectural University.

    Country: JAPAN

    JAPAN Research PublicationJAPAN Research Publication

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    MEDLINETA: J Biochem

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