The Coexistence of Ser84 in Renin and His13 in Angiotensinogen Brings a pH Profile of Two Separate Peaks to the Reaction of Human Renin and Sheep Angiotensinogen.

The Coexistence of Ser84 in Renin and His13 in Angiotensinogen Brings a pH Profile of Two Separate Peaks to the Reaction of Human Renin and Sheep Angiotensinogen. Research Abstract Details 

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The Coexistence of Ser84 in Renin and His13 in Angiotensinogen Brings a pH Profile of Two Separate Peaks to the Reaction of Human Renin and Sheep Angiotensinogen. Abstract Text:

Hideyuki Iwata,Tsutomu Nakagawa,Yuichiro Yoshioka,Kazufumi Kagei,Kenta Imada,Chiaki Nakane,Hiromi Fujita,Fumiaki Suzuki,Yukio Nakamura,Hideyuki Iwata,Tsutomu Nakagawa,Yuichiro Yoshioka,Kazufumi Kagei,Kenta Imada,Chiaki Nakane,Hiromi Fujita,Fumiaki Suzuki,Yukio Nakamura,Hideyuki Iwata,Tsutomu Nakagawa,Yuichiro Yoshioka,Kazufumi Kagei,Kenta Imada,Chiaki Nakane,Hiromi Fujita,Fumiaki Suzuki,Yukio Nakamura,

The pH dependence and kinetics parameters of renin-angiotensinogen reactions were determined using wild-type and S84G mutant human renins and wild-type and H13Y mutant sheep angiotensinogens. It is explained in this report that (i) renin catalyzes acidic and basic reactions of which the optimum pHs are 5.5 and 7.5-8.2 respectively, both of which produce angiotensin I; (ii) Ser84 specific to human renin accelerates the acidic reaction by 75-110% through elevation of V(max), and shifts the optimum pH of the basic reaction from 7.5 to 8.0-8.2; and (iii) His13 specific to sheep angiotensinogen accelerates the acidic and basic reactions by 25-42% through reduction of K(m). It is concluded from these results that the coexistence of Ser84 in renin and His13 in angiotensinogen brings a pH profile of two separate peaks at pHs 5.5 and 8.2 to the reaction of human renin and sheep angiotensinogen.

The Coexistence of Ser84 in Renin and His13 in Angiotensinogen Brings a pH Profile of Two Separate Peaks to the Reaction of Human Renin and Sheep Angiotensinogen. Publishing Authors By Initials

H Iwata,T Nakagawa,Y Yoshioka,K Kagei,K Imada,C Nakane,H Fujita,F Suzuki,Y Nakamura,H Iwata,T Nakagawa,Y Yoshioka,K Kagei,K Imada,C Nakane,H Fujita,F Suzuki,Y Nakamura,H Iwata,T Nakagawa,Y Yoshioka,K Kagei,K Imada,C Nakane,H Fujita,F Suzuki,Y Nakamura,

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The Coexistence of Ser84 in Renin and His13 in Angiotensinogen Brings a pH Profile of Two Separate Peaks to the Reaction of Human Renin and Sheep Angiotensinogen. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Bioscience, biotechnology, and biochemistry

VOLUME: 72

Page Numbers: 179-85

Journal Abbreviation: Biosci. Biotechnol. Biochem.

ISSN: 1347-6947

DAY: 7

MONTH: 01

YEAR: 2008

The Coexistence of Ser84 in Renin and His13 in Angiotensinogen Brings a pH Profile of Two Separate Peaks to the Reaction of Human Renin and Sheep Angiotensinogen. Information

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LANGUAGE: eng

NlmUniqueID: 9205717

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AFFILIATION: United Graduate School of Agricultural Science, Gifu University.

Country: Japan

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MEDLINETA: Biosci Biotechnol Biochem

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