The Clustering of Inositol 1,4,5-Trisphosphate (IP(3)) Receptors Is Triggered by IP(3) Binding and Facilitated by Depletion of the Ca(2+) Store.

The Clustering of Inositol 1,4,5-Trisphosphate (IP(3)) Receptors Is Triggered by IP(3) Binding and Facilitated by Depletion of the Ca(2+) Store. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The Clustering of Inositol 1,4,5-Trisphosphate (IP(3)) Receptors Is Triggered by IP(3) Binding and Facilitated by Depletion of the Ca(2+) Store. Abstract Text:

The inositol 1,4,5-trisphosphate receptors (IP(3)Rs) form clusters following agonist stimulation, but its mechanism remains controversial. In this study, we visualized the clustering of green fluorescent protein (GFP)-tagged type 3 IP(3)R (GFP-IP(3)R3) in cultured living cells using confocal microscopy. Stimulation with ATP evoked GFP-IP(3)R3 clustering not only in cells with replete Ca(2+)-stores but also in cells with depleted Ca(2+) stores. Thapsigargin (ThG) and ionomycin failed to mimic the ATP-induced cluster formation despite the continuous elevation of intracellular Ca(2+) concentration ([Ca(2+)](i)). Application of IP(3) caused GFP-IP(3)R3 clustering in permeabilized cells, and the response was completely inhibited by heparin, a competitive inhibitor of IP(3)R. Experiments using LIBRAv, an IP(3) biosensor, showed that ATP significantly stimulated IP(3) generation even in store-depleted cells. We also found that pretreatment with ThG accelerated or enhanced the ATP-induced clustering in both the presence and absence of extracellular Ca(2+). When permeabilized cells were stimulated with the threshold of IP(3), the GFP-IP(3)R3 clustering clearly occurred in Ca(2+)-free medium but not in Ca(2+)-containing medium. These results strongly support the hypothesis that the agonist-induced clustering of IP(3)R is triggered by IP(3) binding, rather than [Ca(2+)](i) elevation. Although depletion of the Ca(2+) store by itself does not cause the clustering, it may increase the sensitivity of IP(3)R to cluster formation, leading to facilitation of IP(3)-triggered clustering.

The Clustering of Inositol 1,4,5-Trisphosphate (IP(3)) Receptors Is Triggered by IP(3) Binding and Facilitated by Depletion of the Ca(2+) Store. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

The Clustering of Inositol 1,4,5-Trisphosphate (IP(3)) Receptors Is Triggered by IP(3) Binding and Facilitated by Depletion of the Ca(2+) Store. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of pharmacological sciences

VOLUME: 107

Page Numbers: 138-50

Journal Abbreviation: J. Pharmacol. Sci.

ISSN: 1347-8613

DAY: 5

MONTH: 06

YEAR: 2008

The Clustering of Inositol 1,4,5-Trisphosphate (IP(3)) Receptors Is Triggered by IP(3) Binding and Facilitated by Depletion of the Ca(2+) Store. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101167001

The Clustering of Inositol 1,4,5-Trisphosphate (IP(3)) Receptors Is Triggered by IP(3) Binding and Facilitated by Depletion of the Ca(2+) Store. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: The Clustering of Inositol 1,4,5-Trisphosphate (IP(3)) Receptors Is Triggered by IP(3) Binding and Facilitated by Depletion of the Ca(2+) Store. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The Clustering of Inositol 1,4,5-Trisphosphate (IP(3)) Receptors Is Triggered by IP(3) Binding and Facilitated by Depletion of the Ca(2+) Store.

AFFILIATION: Department of Pharmacology, School of Dentistry, Health Sciences University of Hokkaido, Japan.

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Pharmacol Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The Clustering of Inositol 1,4,5-Trisphosphate IP3 Receptors Is Triggered by IP3 Binding and Facilitated by Depletion of the Ca2+ Store Related Publications

• A case of 17 alpha-hydroxylase deficiency syndrome associated with right adrenal tumor.
• Dynamics of a multimode system coupled to multiple heat baths probed by two-dimensional infrared spectroscopy.
• Effects of phase changes on reflection and their wavelength dependence in optical profilometry.
• Effects on macaque embryos of drugs reported or suspected to be teratogenic to humans.
• Exploring a free energy landscape by means of multidimensional infrared and terahertz spectroscopies.
• Floating plastic in the Kuroshio Current area, western North Pacific Ocean.
• Nonequilibrium molecular dynamics simulations with a backward-forward trajectories sampling for multidimensional infrared spectroscopy of molecular vibrational modes.
• Risk factors for falls among blind elderly in a nursing home for the blind.
• The Clustering of Inositol 1,4,5-Trisphosphate (IP(3)) Receptors Is Triggered by IP(3) Binding and Facilitated by Depletion of the Ca(2+) Store.
• Two-dimensional fifth-order Raman spectroscopy of liquid formamide: Experiment and Theory.
• [Romano-Ward syndrome and sympathoganglionectomy (author's transl)]