The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca(2+) dependent manner.

The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca(2+) dependent manner. Research Abstract Details 

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The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca(2+) dependent manner. Abstract Text:

William Urquhart,Arunika H L A N Gunawardena,Wolfgang Moeder,Rashid Ali,Gerald A Berkowitz,Keiko Yoshioka,William Urquhart,Arunika H L A N Gunawardena,Wolfgang Moeder,Rashid Ali,Gerald A Berkowitz,Keiko Yoshioka,William Urquhart,Arunika H L A N Gunawardena,Wolfgang Moeder,Rashid Ali,Gerald A Berkowitz,Keiko Yoshioka,

The hypersensitive response (HR) involves programmed cell death (PCD) in response to pathogen infection. To investigate the pathogen resistance signaling pathway, we previously identified the Arabidopsis mutant cpr22, which displays constitutive activation of multiple defense responses including HR like cell death. The cpr22 mutation has been identified as a 3 kb deletion that fuses two cyclic nucleotide-gated ion channel (CNGC)-encoding genes, ATCNGC11 and ATCNGC12, to generate a novel chimeric gene, ATCNGC11/12. In this study, we conducted a characterization of cell death induced by transient expression of ATCNGC11/12 in Nicotiana benthamiana. Electron microscopic analysis of this cell death showed similar characteristics to PCD, such as plasma membrane shrinkage and vesicle formation. The hallmark of animal PCD, fragmentation of nuclear DNA, was also observed in ATCNGC11/12-induced cell death. The development of cell death was significantly suppressed by caspase-1 inhibitors, suggesting the involvement of caspases in this process. Recently, vacuolar processing enzyme (VPE) was isolated as the first plant caspase-like protein, which is involved in HR development. In VPE-silenced plants development of cell death induced by ATCNGC11/12 was much slower and weaker compared to control plants, suggesting the involvement of VPE as a caspase in ATCNGC11/12-induced cell death. Complementation analysis using a Ca(2+) uptake deficient yeast mutant demonstrated that the ATCNGC11/12 channel is permeable to Ca(2+). Additionally, calcium channel blockers such as GdCl(3) inhibited ATCNGC11/12-induced HR formation, whereas potassium channel blockers did not. Taken together, these results indicate that the cell death that develops in the cpr22 mutant is indeed PCD and that the chimeric channel, ATCNGC11/12, is at the point of, or up-stream of the calcium signal necessary for the development of HR.

The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca(2+) dependent manner. Publishing Authors By Initials

W Urquhart,AH Gunawardena,W Moeder,R Ali,GA Berkowitz,K Yoshioka,W Urquhart,AH Gunawardena,W Moeder,R Ali,GA Berkowitz,K Yoshioka,W Urquhart,AH Gunawardena,W Moeder,R Ali,GA Berkowitz,K Yoshioka,

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The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca(2+) dependent manner. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Plant molecular biology

VOLUME: 65

Page Numbers: 747-61

Journal Abbreviation: Plant Mol. Biol.

ISSN: 0167-4412

DAY: 21

MONTH: 09

YEAR: 2007

The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca(2+) dependent manner. Information

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LANGUAGE: eng

NlmUniqueID: 9106343

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Grant and Affiliation Information for The chimeric cyclic nucleotide-gated ion channel ATCNGC11/12 constitutively induces programmed cell death in a Ca(2+) dependent manner.

AFFILIATION: Department of Cell and Systems Biology, University of Toronto, 25 Willcocks Street, Toronto, ON, Canada, M5S 3B2.

Country: Netherlands

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MEDLINETA: Plant Mol Biol

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