The cellular and genomic response of an immortalized microglia cell line (BV2) to concentrated ambient particulate matter.

The cellular and genomic response of an immortalized microglia cell line (BV2) to concentrated ambient particulate matter. Research Abstract Details 

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The cellular and genomic response of an immortalized microglia cell line (BV2) to concentrated ambient particulate matter. Abstract Text:

Preethi Sama,Thomas C Long,Susan Hester,Julianne Tajuba,Joel Parker,Lung-Chi Chen,Bellina Veronesi,

Ambient particulate matter (PM) damages pulmonary tissue through oxidative stress (OS) pathways. Several reports indicate that the brain is another affected target of PM exposure. Since microglia (brain macrophages) are critical to OS-mediated neurodegeneration, the cellular and genomic response of immortalized mouse microglia (BV2) was examined in response to fine (or= 250 microg/ml) and depolarized mitochondrial membranes (>or= 6 microg/ml) within 15 min of exposure. HP and LP CAPs (>or= 25 microg/ml) differentially affected the endogenous scavengers, glutathione and nonprotein sulfhydryl in BV2 microglia after 1.5 h of exposure. Both HP and LP CAPs stimulated the release of proinflammatory cytokines tumor necrosis factor (TNF) alpha and interleukin (IL)-6 after 6 h of exposures. Microarray analysis of BV2 microglia exposed to either HP or LP CAPs (75 microg/ml, 4 h) identified 3200 (HP CAPs) and 160 (LP CAPs) differentially expressed (up- and downregulated) genes relative to media controls. Of the 3200 genes significantly affected by HP CAPs, the most prominent upregulated gene probes related to inflammatory pathways associated with Toll-like receptor signaling, MAPK signaling, T- and B-cell receptor signaling, apoptosis, and various proinflammatory cytokines and their receptors. LP CAPs significantly affected 160 genes that related to pathways associated with cellular maintenance and division, cell cycling and nuclear events. These data suggest that HP CAPs, which contained higher levels of nickel and vanadium than LP CAPs, appear to be more inflammatory and selectively upregulated the expression of inflammatory and innate immunity pathways in BV2 microglia.

The cellular and genomic response of an immortalized microglia cell line (BV2) to concentrated ambient particulate matter. Publishing Authors By Initials

P Sama,TC Long,S Hester,J Tajuba,J Parker,LC Chen,B Veronesi,

For similar complex mixtures: particulate matter research abstracts see: complex mixtures: particulate matter research

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The cellular and genomic response of an immortalized microglia cell line (BV2) to concentrated ambient particulate matter. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Inhalation toxicology

VOLUME: 19

Page Numbers: 1079-87

Journal Abbreviation:

ISSN: 1091-7691

DAY: 30

MONTH: Oct

YEAR: 2007

The cellular and genomic response of an immortalized microglia cell line (BV2) to concentrated ambient particulate matter. Information

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LANGUAGE: eng

NlmUniqueID: 8910739

The cellular and genomic response of an immortalized microglia cell line (BV2) to concentrated ambient particulate matter. Keywords Mesh Terms:

KEYWORDS: Particulate Matter

MESH TERMS: toxicity

Chemical & Substance for Abstract: The cellular and genomic response of an immortalized microglia cell line (BV2) to concentrated ambient particulate matter. Information

Substance Name: Particulate Matter

Registry Number: 0

Grant and Affiliation Information for The cellular and genomic response of an immortalized microglia cell line (BV2) to concentrated ambient particulate matter.

AFFILIATION: Department of Environmental Science and Engineering, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA.

Country: United States

AGENCY: United States NIEHS

GRANT: R01ES015495

ACRONYM: ES

MEDLINETA: Inhal Toxicol

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