The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency.

The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Research Abstract Details 

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The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Abstract Text:

Sandii N Brimble,Eric S Sherrer,Elizabeth W Uhl,Elaine Wang,Samuel Kelly,Alfred H Merrill,Allan J Robins,Thomas C Schulz,

Pluripotent cells can be isolated from the human blastocyst and maintained in culture as self-renewing, undifferentiated, human ESCs (hESCs). These cells are a valuable model of human development in vitro and are the focus of substantial research aimed at generating differentiated populations for cellular therapies. The extracellular markers that have been used to characterize hESCs are primarily carbohydrate epitopes on proteoglycans or sphingolipids, such as stage-specific embryonic antigen (SSEA)-3 and -4. The expression of SSEA-3 and -4 is tightly regulated during preimplantation development and on hESCs. Although this might imply a molecular function in undifferentiated cells, it has not yet been tested experimentally. We used inhibitors of sphingolipid and glycosphingolipid (GSL) biosynthesis to block the generation of SSEA-3 and -4 in hESCs. Depletion of these antigens and their precursors was confirmed using immunostaining, flow cytometry, and tandem mass spectroscopy. Transcriptional analysis, immunostaining, and differentiation in vitro and in teratomas indicated that other properties of pluripotency were not noticeably affected by GSL depletion. These experiments demonstrated that the GSLs recognized as SSEA-3 and -4 do not play critical functional roles in maintaining the pluripotency of hESCs, but instead suggested roles for this class of molecules during cellular differentiation.

The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Publishing Authors By Initials

SN Brimble,ES Sherrer,EW Uhl,E Wang,S Kelly,AH Merrill,AJ Robins,TC Schulz,

For similar cells: stem cells: pluripotent stem cells research abstracts see: cells: stem cells: pluripotent stem cells research

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The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Stem cells (Dayton, Ohio)

VOLUME: 25

Page Numbers: 54-62

Journal Abbreviation: Stem Cells

ISSN: 1066-5099

DAY: 28

MONTH: 09

YEAR: 2006

The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9304532

The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Keywords Mesh Terms:

KEYWORDS: Pluripotent Stem Cells

MESH TERMS: physiology

Chemical & Substance for Abstract: The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Information

Substance Name: stage-specific embryonic antigen 4

Registry Number: 0

Grant and Affiliation Information for The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency.

AFFILIATION: Novocell, Athens, Georgia, USA.

Country: United States

AGENCY: United States NCRR

GRANT: R24RR021313-05

ACRONYM: RR

MEDLINETA: Stem Cells

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