The cationic C-terminus of rat Muc2 facilitates dimer formation post translationally and is subsequently removed by furin.

The cationic C-terminus of rat Muc2 facilitates dimer formation post translationally and is subsequently removed by furin. Research Abstract Details 

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The cationic C-terminus of rat Muc2 facilitates dimer formation post translationally and is subsequently removed by furin. Abstract Text:

G Xu,S L Bell,D McCool,J F Forstner,

Earlier immunolocalization experiments showed that the extreme cationic C-terminus of the rat intestinal mucin Muc2 (RMC) was present at the base of intestinal goblet cells in the vicinity of ER and golgi compartments, but was not found with the rest of the mucin in apical storage granules. This prompted us to investigate the possibility that an early proteolytic cleavage reaction occurs post-translationally. A plasmid pRMC, encoding the C-terminal 534 amino acids of the mucin, was expressed in COS-7 cells and was shown to undergo cleavage at an R-T-R-R sequence located within the C-terminal 14 amino acids. Cleavage did not occur with the construct RMCfH, a furin site-mutated (A-T-A-A) counterpart of pRMCH (poly His6 tagged RMC). Addition of a furin inhibitor to COS-7 cell incubations also prevented cleavage of RMC and RMCH products. 35S pulse-chase kinetic experiments revealed that a truncated mutant lacking the C-terminal 14 amino acids (pRMCDeltaCT) forms faulty (doublet) dimers in the ER. These were not secreted as efficiently as the normal dimer of wild-type (pRMC) constructs. Thus the cationic C-terminus of rMuc2 apppears to facilitate the correct formation of normal Muc2 domain dimers.

The cationic C-terminus of rat Muc2 facilitates dimer formation post translationally and is subsequently removed by furin. Publishing Authors By Initials

G Xu,SL Bell,D McCool,JF Forstner,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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The cationic C-terminus of rat Muc2 facilitates dimer formation post translationally and is subsequently removed by furin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: European journal of biochemistry / FEBS

VOLUME: 267

Page Numbers: 2998-3004

Journal Abbreviation: Eur. J. Biochem.

ISSN: 0014-2956

DAY: 30

MONTH: May

YEAR: 2000

The cationic C-terminus of rat Muc2 facilitates dimer formation post translationally and is subsequently removed by furin. Information

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LANGUAGE: eng

NlmUniqueID: 107600

The cationic C-terminus of rat Muc2 facilitates dimer formation post translationally and is subsequently removed by furin. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: metabolism

Chemical & Substance for Abstract: The cationic C-terminus of rat Muc2 facilitates dimer formation post translationally and is subsequently removed by furin. Information

Substance Name: Furin

Registry Number: EC 3.4.21.75

Grant and Affiliation Information for The cationic C-terminus of rat Muc2 facilitates dimer formation post translationally and is subsequently removed by furin.

AFFILIATION: Research Institute, The Hospital for Sick Children and the University of Toronto, Canada.

Country: GERMANY

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MEDLINETA: Eur J Biochem

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