The carboxyl-terminus directs TAF(I)48 to the nucleus and nucleolus and associates with multiple nuclear import receptors.

The carboxyl-terminus directs TAF(I)48 to the nucleus and nucleolus and associates with multiple nuclear import receptors. Research Abstract Details 

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The carboxyl-terminus directs TAF(I)48 to the nucleus and nucleolus and associates with multiple nuclear import receptors. Abstract Text:

Joseph L Dynes,Shuping Xu,Sarah Bothner,Jill M Lahti,Roderick T Hori,

The protein complex Selectivity Factor 1, composed of TBP, TAF(I)48, TAF(I)63 and TAF(I)110, is required for rRNA transcription by RNA polymerase I in the nucleolus. The steps involved in targeting Selectivity Factor 1 will be dependent on the transport pathways that are used and the localization signals that direct this trafficking. In order to investigate these issues, we characterized human TAF(I)48, a subunit of Selectivity Factor 1. By domain analysis of TAF(I)48, the carboxyl-terminal 51 residues were found to be required for the localization of TAF(I)48, as well as sufficient to direct Green Fluorescent Protein to the nucleus and nucleolus. The carboxyl-terminus of TAF(I)48 also has the ability to associate with multiple members of the beta-karyopherin family of nuclear import receptors, including importin beta (karyopherin beta1), transportin (karyopherin beta2) and RanBP5 (karyopherin beta3), in a Ran-dependent manner. This property of interacting with multiple beta-karyopherins has been previously reported for the nuclear localization signals of some ribosomal proteins that are likewise directed to the nucleolus. This study identifies the first nuclear import sequence identified within the TBP-Associated Factor subunits of Selectivity Factor 1.

The carboxyl-terminus directs TAF(I)48 to the nucleus and nucleolus and associates with multiple nuclear import receptors. Publishing Authors By Initials

JL Dynes,S Xu,S Bothner,JM Lahti,RT Hori,

For similar proteins: carrier proteins: membrane transport proteins: nucleocytoplasmic transport proteins: karyopherins: beta karyopherins research abstracts see: proteins: carrier proteins: membrane transport proteins: nucleocytoplasmic transport proteins: karyopherins: beta karyopherins research

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The carboxyl-terminus directs TAF(I)48 to the nucleus and nucleolus and associates with multiple nuclear import receptors. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of biochemistry

VOLUME: 135

Page Numbers: 429-38

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 2004

The carboxyl-terminus directs TAF(I)48 to the nucleus and nucleolus and associates with multiple nuclear import receptors. Information

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LANGUAGE: eng

NlmUniqueID: 376600

The carboxyl-terminus directs TAF(I)48 to the nucleus and nucleolus and associates with multiple nuclear import receptors. Keywords Mesh Terms:

KEYWORDS: beta Karyopherins

MESH TERMS: metabolism

Chemical & Substance for Abstract: The carboxyl-terminus directs TAF(I)48 to the nucleus and nucleolus and associates with multiple nuclear import receptors. Information

Substance Name: beta Karyopherins

Registry Number: 0

Grant and Affiliation Information for The carboxyl-terminus directs TAF(I)48 to the nucleus and nucleolus and associates with multiple nuclear import receptors.

AFFILIATION: Reeve-Irvine Research Center, Department of Anatomy and Neurobiology, University of California, Irvine, CA 92697, USA.

Country: Japan

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MEDLINETA: J Biochem

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