The C-terminal domain of T4 RNA ligase 1 confers specificity for tRNA repair.

The C-terminal domain of T4 RNA ligase 1 confers specificity for tRNA repair. Research Abstract Details 

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The C-terminal domain of T4 RNA ligase 1 confers specificity for tRNA repair. Abstract Text:

Li Kai Wang,Jayakrishnan Nandakumar,Beate Schwer,Stewart Shuman,

T4 RNA ligase 1 (Rnl1) is a tRNA repair enzyme that thwarts a tRNA-damaging host response to virus infection. The 374-aa Rnl1 protein consists of an N-terminal nucleotidyltransferase domain fused to a unique C-terminal domain composed of 10 alpha helices. We exploited an in vitro tRNA splicing system to demonstrate that Rnl1 has an inherent specificity for sealing tRNA with a break in the anticodon loop. The tRNA specificity is imparted by the C domain, any deletion of which caused the broken tRNA to be sealed as poorly as the linear intron in vitro and also abolished Rnl1 tRNA splicing activity in vivo. Deletion analysis demarcated Rnl1-(1-254) as a minimal catalytic domain of Rnl1, capable of all chemical steps of the nonspecific RNA ligation reaction. Alanine scanning of the N domain identified Ser103, Leu104, Lys117, and Ser118 as important for pRNA ligation in vitro and tRNA repair in vivo.

The C-terminal domain of T4 RNA ligase 1 confers specificity for tRNA repair. Publishing Authors By Initials

LK Wang,J Nandakumar,B Schwer,S Shuman,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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MEDLINE DATE:

The C-terminal domain of T4 RNA ligase 1 confers specificity for tRNA repair. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: RNA (New York, N.Y.)

VOLUME: 13

Page Numbers: 1235-44

Journal Abbreviation: RNA

ISSN: 1355-8382

DAY: 21

MONTH: 06

YEAR: 2007

The C-terminal domain of T4 RNA ligase 1 confers specificity for tRNA repair. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9509184

The C-terminal domain of T4 RNA ligase 1 confers specificity for tRNA repair. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: The C-terminal domain of T4 RNA ligase 1 confers specificity for tRNA repair. Information

Substance Name: bacteriophage T4 RNA ligase 1

Registry Number: EC 6.5.1.3

Grant and Affiliation Information for The C-terminal domain of T4 RNA ligase 1 confers specificity for tRNA repair.

AFFILIATION: Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10021, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM42498

ACRONYM: GM

MEDLINETA: RNA

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