The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.
The blasticidin S resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome. Publishing Authors By Initials
The blasticidin S resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Journal of biochemistry
VOLUME: 107
Page Numbers: 799-801
Journal Abbreviation: J. Biochem.
ISSN: 0021-924X
DAY: 19
MONTH: Jun
YEAR: 1990
The blasticidin S resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome. Information
Number of References:
LANGUAGE: eng
NlmUniqueID: 376600
The blasticidin S resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome. Keywords Mesh Terms:
KEYWORDS: Transformation, Bacterial
MESH TERMS: pharmacology
Chemical & Substance for Abstract: The blasticidin S resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome. Information
Substance Name: blasticidin S
Registry Number: 2079-00-7
Grant and Affiliation Information for The blasticidin S resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome.
AFFILIATION: Mitsubishi Kasei Institute of Life Sciences, Tokyo.
Country: JAPAN
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MEDLINETA: J Biochem
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