The Bead blot: a method for selecting small molecule ligands for protein capture and purification.

The Bead blot: a method for selecting small molecule ligands for protein capture and purification. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The Bead blot: a method for selecting small molecule ligands for protein capture and purification. Abstract Text:

Julia Tait Lathrop,David Hammond,Julia Tait Lathrop,David Hammond,Julia Tait Lathrop,David Hammond,

We present a new method for selecting peptide ligands that are useful for protein purification, protein targeting and exploring protein-ligand interactions, and which requires no prior protein purification or derivatization. In the Bead blot, a complex mixture containing the target protein, for example, plasma, is incubated with a combinatorial library of peptide ligands synthesized on beads. The proteins are fractionated and purified on their respective ligands and the beads with their bound proteins are immobilized in a gel. The proteins are eluted from the ligands by capillary action and captured on a membrane so that their position on the membrane corresponds to the position of the beads in the gel. The protein is detected on the membrane, generating spots on an autoradiography film, the spots on the film are aligned with the beads in the gel, and the beads that bound the protein are recovered. The ligand on the bead(s) can be sequenced and synthesized at large scale for protein purification. The Bead blot can be completed in several hours with overnight pause steps if desired. On average, 5 prospective ligands are selected per 50,000 beads screened using this method. Unlike other ligand identification methods, the target protein does not have to be purified or labeled, and the Bead blot allows ligands for multiple proteins to be selected in a single experiment.

The Bead blot: a method for selecting small molecule ligands for protein capture and purification. Publishing Authors By Initials

JT Lathrop,D Hammond,JT Lathrop,D Hammond,JT Lathrop,D Hammond,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

The Bead blot: a method for selecting small molecule ligands for protein capture and purification. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nature protocols

VOLUME: 2

Page Numbers: 3102-10

Journal Abbreviation:

ISSN: 1750-2799

DAY: 14

MONTH: 12

YEAR: 2007

The Bead blot: a method for selecting small molecule ligands for protein capture and purification. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101284307

The Bead blot: a method for selecting small molecule ligands for protein capture and purification. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: The Bead blot: a method for selecting small molecule ligands for protein capture and purification. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The Bead blot: a method for selecting small molecule ligands for protein capture and purification.

AFFILIATION: Prolias, LLC, Rockville, Maryland 20855, USA. julia.lathrop@prolias.us

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Nat Protoc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The Bead blot: a method for selecting small molecule ligands for protein capture and purification Related Publications

• A classification of handedness using the Annett Hand Preference Questionnaire.
• Adenylyl cyclase gene transfer in heart failure.
• Adenylylcyclase gene transfer increases function of the failing heart.
• Androstene-3,5-dienes as ER-beta selective SERMs.
• Axial patterning in the developing vertebrate inner ear.
• Cell-based GATA4 cardiac gene transfer using cell-penetrating peptide.
• Combined oral contraceptives: a comprehensive review.
• Diamagnetic levitation changes growth, cell cycle, and gene expression of Saccharomyces cerevisiae.
• Editorial--Individuation through socialization.
• Effect of burn injury on corticosteroid-binding globulin levels in plasma and wound fluid.
• Food at end of life.
• Intramolecular hydroamination of difluoropropargyl amides: regioselective synthesis of fluorinated beta- and gamma-lactams.
• Large scale synthesis of the Cdc42 inhibitor secramine A and its inhibition of cell spreading.
• Mechanisms responsible for the enhanced antinociceptive effects of micro-opioid receptor agonists in the rostral ventromedial medulla of male rats with persistent inflammatory pain.
• MicroRNAs as tumor suppressors.
• Microarray Analysis of miRNA Gene Expression.
• New signaling pathways associated with increased cardiac adenylyl cyclase 6 expression: implications for possible congestive heart failure therapy.
• Ovarian mucinous tumour arising in mature cystic teratoma and associated with pseudomyxoma peritonei: report of two cases and comparison with ovarian involvement by low-grade appendiceal mucinous tumour.
• Promoting the peer-related social development of young children with mild developmental delays: effectiveness of a comprehensive intervention.
• Selective synthesis of fluorinated furan derivatives via AgNO3-catalyzed activation of an electronically deficient triple bond.
• Sex hormone-binding globulin expression in sea bass (Dicentrarchus labrax L.) throughout development and the reproductive season.
• Suitability of the retrograde tracer Dil for electrophysiological studies of brainstem neurons: adverse ramifications for G-protein coupled receptor agonists.
• Testicular, semen and blood parameters in adapted and nonadapted Bos taurus bulls in the semi-arid tropics.
• The Bead blot: a method for selecting small molecule ligands for protein capture and purification.
• The TATA binding protein associated factor 4b (TAF4b) mediates FSH stimulation of the IGFBP-3 promoter in cultured porcine ovarian granulosa cells.
• The cost-effectiveness of pharmacist-led treatment of cardiac risk in patients with type 2 diabetes.
• The glare hypothesis of macular pigment function.
• Therans-3-enoic acids ofAster alpinus andArctium minus seed oils.
• Three-dimensional face shape in Fabry disease.
• microRNA expression in the prefrontal cortex of individuals with schizophrenia and schizoaffective disorder.