The BBXB motif of RANTES is the principal site for heparin binding and controls receptor selectivity.

The BBXB motif of RANTES is the principal site for heparin binding and controls receptor selectivity. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The BBXB motif of RANTES is the principal site for heparin binding and controls receptor selectivity. Abstract Text:

A E Proudfoot,S Fritchley,F Borlat,J P Shaw,F Vilbois,C Zwahlen,A Trkola,D Marchant,P R Clapham,T N Wells,

The chemokine RANTES (regulated on activation normal T cell expressed and secreted; CCL5) binds selectively to glycosaminoglycans (GAGs) such as heparin, chondroitin sulfate, and dermatan sulfate. The primary sequence of RANTES contains two clusters of basic residues, (44)RKNR(47) and (55)KKWVR(59). The first is a BBXB motif common in heparin-binding proteins, and the second is located in the loop directly preceding the C-terminal helix. We have mutated these residues to alanine, both as point mutations as well as triple mutations of the 40s and 50s clusters. Using a binding assay to heparin beads with radiolabeled proteins, the (44)AANA(47) mutant demonstrated an 80% reduction in its capacity to bind heparin, whereas the (55)AAWVA(59) mutant retained full binding capacity. Mutation of the (44)RKNR(47) site reduced the selectivity of RANTES binding to different GAGs. The mutants were tested for their integrity by receptor binding assays on CCR1 and CCR5 as well as their ability to induce chemotaxis in vitro. In all assays the single point mutations and the triple 50s cluster mutation caused no significant difference in activity compared with the wild type sequence. However, the triple 40s mutant showed a 80-fold reduction in affinity for CCR1, despite normal binding to CCR5. It was only able to induce monocyte chemotaxis at micromolar concentrations. The triple 40s mutant was also able to inhibit HIV-1 infectivity, but consistent with its abrogated GAG binding capacity, it no longer induced enhanced infectivity at high concentrations.

The BBXB motif of RANTES is the principal site for heparin binding and controls receptor selectivity. Publishing Authors By Initials

AE Proudfoot,S Fritchley,F Borlat,JP Shaw,F Vilbois,C Zwahlen,A Trkola,D Marchant,PR Clapham,TN Wells,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

PUBMED ID PMID:

MEDLINE DATE:

The BBXB motif of RANTES is the principal site for heparin binding and controls receptor selectivity. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of biological chemistry

VOLUME: 276

Page Numbers: 10620-6

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 14

MONTH: 12

YEAR: 2000

The BBXB motif of RANTES is the principal site for heparin binding and controls receptor selectivity. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

The BBXB motif of RANTES is the principal site for heparin binding and controls receptor selectivity. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: metabolism

Chemical & Substance for Abstract: The BBXB motif of RANTES is the principal site for heparin binding and controls receptor selectivity. Information

Substance Name: Heparin

Registry Number: 9005-49-6

Grant and Affiliation Information for The BBXB motif of RANTES is the principal site for heparin binding and controls receptor selectivity.

AFFILIATION: Serono Pharmaceutical Research Institute, 14 Chemin des Aulx, 1228 Plan-les-Ouates, Geneva, Switzerland. amanda.proudfoot@serono.com

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The BBXB motif of RANTES is the principal site for heparin binding and controls receptor selectivity Related Publications

• Amino-terminally modified RANTES analogues demonstrate differential effects on RANTES receptors.
• Antimicrobial activities of silver used as a polymerization catalyst for a wound-healing matrix.
• Bad nights or bad bars? Multi-level analysis of environmental predictors of aggression in late-night large-capacity bars and clubs.
• Bayesian population modeling of effective connectivity.
• Cervical cancer and human papillomaviruses: inactivation of retinoblastoma and other tumor suppressor pathways.
• Clinical content detection for medical image retrieval.
• Clinicians' perspectives on cognitive-behavioral treatment for PTSD among persons with severe mental illness.
• Detailed investigation of factors influencing amplification efficiency and allele drop-out in single cell PCR: implications for preimplantation genetic diagnosis.
• Differences in club drug use between heterosexual and lesbian/bisexual females.
• Double-strand breaks in the myotonic dystrophy type 1 and the fragile X syndrome triplet repeat sequences induce different types of mutations in DNA flanking sequences in Escherichia coli.
• Extension of recombinant human RANTES by the retention of the initiating methionine produces a potent antagonist.
• Heparan sulfate proteoglycans mediate Staphylococcus aureus interactions with intestinal epithelium.
• Hot-spot mimicry of a cytokine receptor by a small molecule.
• Image reconstruction of effective Mie scattering parameters of breast tissue in vivo with near-infrared tomography.
• Influence of patient preference and primary care clinician proclivity for watchful waiting on receipt of depression treatment.
• Logarithm odds maps for shape representation.
• Mental disorder comorbidity in Te Rau Hinengaro: the New Zealand Mental Health Survey.
• Mental health care for adults with suicide ideation.
• Mental-physical comorbidity in Te Rau Hinengaro: the New Zealand Mental Health Survey.
• Molecular basis of vertebrate endoderm development.
• Novel three-dimensional organotypic liver bioreactor to directly visualize early events in metastatic progression.
• Post-thrombotic syndrome, functional disability and quality of life after upper extremity deep venous thrombosis in adults.
• The BBXB motif of RANTES is the principal site for heparin binding and controls receptor selectivity.
• The cyclin-dependent kinase inhibitor p27kip1 is required for transplantation tolerance induced by costimulatory blockade.
• The involvement of non-B DNA structures in gross chromosomal rearrangements.
• Transcriptional regulation by Foxp3 is associated with direct promoter occupancy and modulation of histone acetylation.
• Treatment failure in emergency department patients with cellulitis.
• Triacylglycerol-rich lipophorins are found in the dipteran infraorder Culicomorpha, not just in mosquitoes.
• Validation of image segmentation by estimating rater bias and variance.
• What is necessary to transform the quality of mental health care.