The bacteriophage lambdaQ anti-terminator protein regulates late gene expression as a stable component of the transcription elongation complex.

The bacteriophage lambdaQ anti-terminator protein regulates late gene expression as a stable component of the transcription elongation complex. Research Abstract Details 

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The bacteriophage lambdaQ anti-terminator protein regulates late gene expression as a stable component of the transcription elongation complex. Abstract Text:

Padraig Deighan,Ann Hochschild,

The Q protein of bacteriophage lambda (lambdaQ) is a transcription anti-terminator required for the expression of the phage's late genes under the control of promoter P(R'). To effect terminator read-through, lambdaQ must gain access to RNA polymerase (RNAP) via a promoter-restricted pathway. In particular, lambdaQ modifies RNAP by binding a specific DNA site embedded in P(R') and interacting with RNAP in the context of a specific paused early elongation complex. The resultant lambdaQ-modified transcription elongation complex is competent to read through downstream termination signals. Here we use a chromatin-immunoprecipitation assay to test the hypothesis that lambdaQ functions as a stable component of the transcription elongation complex. Our results indicate that, in vivo, the lambdaQ-modified transcription elongation complex contains Q as a stably associated subunit. Furthermore, we find that in the physiologically relevant context of an induced lambda lysogen, Q remains stably associated with RNAP as it transcribes at least 22 kb of the phage late operon. Thus, our findings suggest that the promoter-specific pathway leading to lambdaQ-mediated terminator read-through results in the formation of a highly stable lambdaQ-containing transcription elongation complex capable of traversing the entire late operon.

The bacteriophage lambdaQ anti-terminator protein regulates late gene expression as a stable component of the transcription elongation complex. Publishing Authors By Initials

P Deighan,A Hochschild,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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The bacteriophage lambdaQ anti-terminator protein regulates late gene expression as a stable component of the transcription elongation complex. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular microbiology

VOLUME: 63

Page Numbers: 911-20

Journal Abbreviation: Mol. Microbiol.

ISSN: 0950-382X

DAY: 3

MONTH: Feb

YEAR: 2007

The bacteriophage lambdaQ anti-terminator protein regulates late gene expression as a stable component of the transcription elongation complex. Information

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LANGUAGE: eng

NlmUniqueID: 8712028

The bacteriophage lambdaQ anti-terminator protein regulates late gene expression as a stable component of the transcription elongation complex. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: The bacteriophage lambdaQ anti-terminator protein regulates late gene expression as a stable component of the transcription elongation complex. Information

Substance Name: Viral Proteins

Registry Number: 0

Grant and Affiliation Information for The bacteriophage lambdaQ anti-terminator protein regulates late gene expression as a stable component of the transcription elongation complex.

AFFILIATION: Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM44025

ACRONYM: GM

MEDLINETA: Mol Microbiol

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