The assessment of circulating 25(OH)D and 1,25(OH)2D: where we are and where we are going.

The assessment of circulating 25(OH)D and 1,25(OH)2D: where we are and where we are going. Research Abstract Details 

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The assessment of circulating 25(OH)D and 1,25(OH)2D: where we are and where we are going. Abstract Text:

Bruce W Hollis,Ronald L Horst,

The field of Vitamin D assay technology has progressed significantly over the past 4 decades. Further, the clinical utility of these measurements has moved from esoteric into mainstream clinical diagnosis. This movement has been fueled by the realization that Vitamin D is involved in bodily systems beyond skeletal integrity. The clinical assay techniques for circulating 25(OH)D and 1,25(OH)(2)D have progressed away from competitive protein binding assay (CPBAs) that utilize tritium reporters to radioimmunoassay (RIAs) that utilize both I(125) and chemiluminescent reporters. These advances have allowed direct serum analysis of 25(OH)D in an automated format that provides a huge sample throughput. Detection of circulating 25(OH)D can also be achieved utilizing direct high-performance liquid chromatographic (HPLC) or liquid chromatography coupled with mass spectrometry (LC-MS) techniques. These methods are accurate, however, they require expensive equipment and restrict sample throughput in the large clinical laboratory. Direct serum detection of 1,25(OH)(2)D is unlikely to occur for many reasons as a sample pre-purification will always be required. However, a semi-automated chemiluminescent detection system with automated sample preparation is in final development for the determination of circulating 1,25(OH)(2)D. These advances will allow both 25(OH)D and 1,25(OH)(2)D to be detected in an accurate, rapid fashion to meet the clinical demands we see emerging.

The assessment of circulating 25(OH)D and 1,25(OH)2D: where we are and where we are going. Publishing Authors By Initials

BW Hollis,RL Horst,

For similar polycyclic compounds: steroids: cholestanes: cholestenes: cholecalciferol: hydroxycholecalciferols research abstracts see: polycyclic compounds: steroids: cholestanes: cholestenes: cholecalciferol: hydroxycholecalciferols research

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The assessment of circulating 25(OH)D and 1,25(OH)2D: where we are and where we are going. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of steroid biochemistry and molecular

VOLUME: 103

Page Numbers: 473-6

Journal Abbreviation: J. Steroid Biochem. Mol. Biol.

ISSN: 0960-0760

DAY: 2

MONTH: 01

YEAR: 2007

The assessment of circulating 25(OH)D and 1,25(OH)2D: where we are and where we are going. Information

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LANGUAGE: eng

NlmUniqueID: 9015483

The assessment of circulating 25(OH)D and 1,25(OH)2D: where we are and where we are going. Keywords Mesh Terms:

KEYWORDS: Hydroxycholecalciferols

MESH TERMS: metabolism

Chemical & Substance for Abstract: The assessment of circulating 25(OH)D and 1,25(OH)2D: where we are and where we are going. Information

Substance Name: Hydroxycholecalciferols

Registry Number: 0

Grant and Affiliation Information for The assessment of circulating 25(OH)D and 1,25(OH)2D: where we are and where we are going.

AFFILIATION: Departments of Pediatrics, Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA. hollisb@musc.edu

Country: England

AGENCY: United States NICHD

GRANT: R01 HD047511-01A2

ACRONYM: HD

MEDLINETA: J Steroid Biochem Mol Biol

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