The application of droplet counter-current chromatography (DCC) for the separation of acidic glycolipids.

The application of droplet counter-current chromatography (DCC) for the separation of acidic glycolipids. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The application of droplet counter-current chromatography (DCC) for the separation of acidic glycolipids. Abstract Text:

H Otsuka,T Yamakawa,

Droplet counter-current chromatography (DCC) a novel liquid-liquid partition technique, is a useful method for the separation of glycolipids. After a series of investigations on suitable experimental conditions for DDC, mouse brain chloroform-methanol-0.5% CaCl2-n-propanol (50:60:40:6) by the ascending method with 500 standard columns. GM1 and cerebroside sulfuric ester (CSE) from whale brain glycolipid fraction were separated by DCC into two peaks and three peaks, respectively, with chloroform-methanol-water-n-propanol (50:60:40:2) and 500 standard columns. The occurrence of two peaks of GM1 was due to the differences of sphingenine and fatty acid compositions. In the former peak of GM1, the majority of long chain base and fatty acid were 18:1 sphingenine and stearic acid, respectively, while the latter contained 18:1 (32%), 20:1 (68%) sphingenine and 16:0 (14.5%), 18:0 (61.4%), and 20:0 (21.0%) fatty acids. The three peaks of CSE were due to the difference of carbon chain length and to the presence or absence of a hydroxyl group in fatty acids. The first polar fraction (CSE-I) had hydroxy 23:0 (34%) and 24.1 (53.4%) fatty acids and the second fraction (CSE-II) contained hydroxy 24:0 (70.5%) and 25:1 (12.8%) fatty acids, while the third fraction (CSE-III) had normal 23:0 (11.9%), 24:1 (56.4%), hydroxy 25:0 (11.4%) and 26:1 (9.7%) fatty acids. Preparative DCC was also applied to the glycolipid fraction from Tay-Sachs' brain to give 242 mg of practically pure GM2 from 700 mg of crude brain extract without any preliminary purification.

The application of droplet counter-current chromatography (DCC) for the separation of acidic glycolipids. Publishing Authors By Initials

H Otsuka,T Yamakawa,

For similar animals: chordata: vertebrates: mammals: cetacea: whales research abstracts see: animals: chordata: vertebrates: mammals: cetacea: whales research

PUBMED ID PMID:

MEDLINE DATE:

The application of droplet counter-current chromatography (DCC) for the separation of acidic glycolipids. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 90

Page Numbers: 247-54

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1981

The application of droplet counter-current chromatography (DCC) for the separation of acidic glycolipids. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

The application of droplet counter-current chromatography (DCC) for the separation of acidic glycolipids. Keywords Mesh Terms:

KEYWORDS: Whales

MESH TERMS: analysis

Chemical & Substance for Abstract: The application of droplet counter-current chromatography (DCC) for the separation of acidic glycolipids. Information

Substance Name: Hydroxy Acids

Registry Number: 0

Grant and Affiliation Information for The application of droplet counter-current chromatography (DCC) for the separation of acidic glycolipids.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The application of droplet counter-current chromatography DCC for the separation of acidic glycolipids Related Publications

• Absence of seminolipid in seminoma tissue with concomitant increase of sphingoglycolipids.
• Antigenic properties of a synthetic protein complex with glycolipids and related substances.
• Application of trifluoroacetyl derivatives to sugar and lipid chemistry. I. Gas chromatographic analysis of common constituents of glycolipids.
• Application of trifluoroacetyl derivatives to sugar and lipid chemistry. II. Gas chromatographic quantitation of methyl glycosides.
• Biosynthesis of seminolipid: sulfation in vivo and in vitro.
• Blood group A activities of glycoprotein and glycolipid from human erythrocyte membranes.
• Ceramide induces apoptosis in human lung adenocarcinoma A549 cells through mitogen-activated protein kinases.
• Chemical structure of glycolipid of guinea pig red blood cell membrane.
• Chemical structure of oligosaccharides in the blue-green alga, Tolypothrix tenuis.
• Chemistry of lipid of the posthemyolytic residue or stroma of erythrocytes. XVI. Occurrence of ceramide pentasaccharide in the membrane of erythrocytes and reticulocytes of rabbit.
• Existence of glucosaminyl lactosyl ceramide (amino CTH-I) in human erythrocyte membranes as a possible precursor of blood group-active glycolipids.
• Expression of antimicrobial peptide genes encoding Enbocin and Gloverin isoforms in the silkworm, Bombyx mori.
• Fast reconstruction of tissue elastic modulus image by ultrasound.
• Glycolipids of the fish testis.
• Glycosyl glycerides from Streptococcus hemolyticus strain D-58.
• Human kidney alpha-galactosidase. Multiplicity and enzyme activities for ceramide trihexoside and some aryl alpha-galactosides.
• Identification and functional analysis of Relish homologs in the silkworm, Bombyx mori.
• Isolation and characterization of a ganglioside containing fucose from boar testis.
• Isolation and characterization of a novel sulfoglycolipid, 'seminolipid,' from boar testis and spermatozoa.
• Isolation of cerebroside containing glucose (Glucosyl ceramide) and its possible significance in ganglioside synthesis.
• Multiple components of beta-N-acetylhexosaminidase from equine kidney. Their action on glycolipids and allied oligosaccharides.
• On the nature of so-called ungulic acid.
• Presence of alpha-anomeric glycosidic configuration in the glycolipids accumulated in kidney with Fabry's disease.
• Purification of arylsulfatase A from boar testis and its activities toward seminolipid and sulfatide.
• Relationships among the multiple components of beta-N-acetylhexosaminidase from equine kidney.
• Separation of polar glycolipids from human red blood cells with special reference to blood group-A activity.
• Structural study on tetraglycosyl ceremide and gangliosides isolated from human red blood cells.
• The structure and formation mechanism of N-acetone derivatives of phosphatidyl ethanolamine.
• The susceptibility of some complex sphingoglycolipids to -N-acetylhexosaminidase from hog epididymis.
• [Bidirectional ventricular tachycardia]