The antiproliferative effects of agmatine correlate with the rate of cellular proliferation.

The antiproliferative effects of agmatine correlate with the rate of cellular proliferation. Research Abstract Details 

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The antiproliferative effects of agmatine correlate with the rate of cellular proliferation. Abstract Text:

Masato Isome,Mark J Lortie,Yasuko Murakami,Eva Parisi,Senya Matsufuji,Joseph Satriano,

Polyamines are small cationic molecules required for cellular proliferation. Agmatine is a biogenic amine unique in its capacity to arrest proliferation in cell lines by depleting intracellular polyamine levels. We previously demonstrated that agmatine enters mammalian cells via the polyamine transport system. As polyamine transport is positively correlated with the rate of cellular proliferation, the current study examines the antiproliferative effects of agmatine on cells with varying proliferative kinetics. Herein, we evaluate agmatine transport, intracellular accumulation, and its effects on antizyme expression and cellular proliferation in nontransformed cell lines and their transformed variants. H-ras- and Src-transformed murine NIH/3T3 cells (Ras/3T3 and Src/3T3, respectively) that were exposed to exogenous agmatine exhibit increased uptake and intracellular accumulation relative to the parental NIH/3T3 cell line. Similar increases were obtained for human primary foreskin fibroblasts relative to a human fibrosarcoma cell line, HT1080. Agmatine increases expression of antizyme, a protein that inhibits polyamine biosynthesis and transport. Ras/3T3 and Src/3T3 cells demonstrated augmented increases in antizyme protein expression relative to NIH/3T3 in response to agmatine. All transformed cell lines were significantly more sensitive to the antiproliferative effects of agmatine than nontransformed lines. These effects were attenuated in the presence of exogenous polyamines or inhibitors of polyamine transport. In conclusion, the antiproliferative effects of agmatine preferentially target transformed cell lines due to the increased agmatine uptake exhibited by cells with short cycling times.

The antiproliferative effects of agmatine correlate with the rate of cellular proliferation. Publishing Authors By Initials

M Isome,MJ Lortie,Y Murakami,E Parisi,S Matsufuji,J Satriano,

For similar organic chemicals: amines: biogenic amines: biogenic polyamines: putrescine research abstracts see: organic chemicals: amines: biogenic amines: biogenic polyamines: putrescine research

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The antiproliferative effects of agmatine correlate with the rate of cellular proliferation. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: American journal of physiology. Cell physiology

VOLUME: 293

Page Numbers: C705-11

Journal Abbreviation: Am. J. Physiol., Cell Physiol.

ISSN: 0363-6143

DAY: 2

MONTH: 05

YEAR: 2007

The antiproliferative effects of agmatine correlate with the rate of cellular proliferation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901225

The antiproliferative effects of agmatine correlate with the rate of cellular proliferation. Keywords Mesh Terms:

KEYWORDS: Putrescine

MESH TERMS: metabolism

Chemical & Substance for Abstract: The antiproliferative effects of agmatine correlate with the rate of cellular proliferation. Information

Substance Name: Ornithine Decarboxylase

Registry Number: EC 4.1.1.17

Grant and Affiliation Information for The antiproliferative effects of agmatine correlate with the rate of cellular proliferation.

AFFILIATION: University of California San Diego and Veterans Affairs San Diego Healthcare System, Division of Nephrology-Hypertension, San Diego, CA 92161, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK56248

ACRONYM: DK

MEDLINETA: Am J Physiol Cell Physiol

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