The Anabaena sp. strain PCC 7120 asr1734 gene encodes a negative regulator of heterocyst development.

The Anabaena sp. strain PCC 7120 asr1734 gene encodes a negative regulator of heterocyst development. Research Abstract Details 

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The Anabaena sp. strain PCC 7120 asr1734 gene encodes a negative regulator of heterocyst development. Abstract Text:

Xiaoqiang Wu,Dong W Lee,Rodrigo A Mella,James W Golden,

The novel asr1734 gene of Anabaena (Nostoc) sp. strain PCC 7120 inhibited heterocyst development when present in extra copies. Overexpression of asr1734 inhibited heterocyst development in several strains including the wild type and two strains that form multiple contiguous heterocysts (Mch phenotype): a PatS null mutant and a hetR(R223W) mutant. Overexpression of asr1734 also caused increased nblA messenger RNA levels, and increased loss of autofluorescence in vegetative cells throughout filaments after nitrogen or sulphur depletion. Unlike the wild type, an asr1734 knockout mutant formed 5% heterocysts after a nitrogen shift from ammonium to nitrate, and formed 15% heterocysts and a weak Mch phenotype after step-down to medium lacking combined nitrogen. After nitrogen step-down, the asr1734 mutant had elevated levels of ntcA messenger RNA. A green fluorescent protein reporter driven by the asr1734 promoter, P(asr1734)-gfp, was expressed specifically in differentiating proheterocysts and heterocysts after nitrogen step-down. Strains overexpressing asr1734 and containing P(hetR)-gfp or P(patS)-gfp reporters failed to show normal patterned upregulation 24 h after nitrogen step-down even though hetR expression was upregulated at 6 h. Apparent orthologues of asr1734 are found only in two other filamentous nitrogen-fixing cyanobacteria, Anabaena variabilis and Nostoc punctiforme.

The Anabaena sp. strain PCC 7120 asr1734 gene encodes a negative regulator of heterocyst development. Publishing Authors By Initials

X Wu,DW Lee,RA Mella,JW Golden,

For similar inorganic chemicals: elements: chalcogens: sulfur research abstracts see: inorganic chemicals: elements: chalcogens: sulfur research

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The Anabaena sp. strain PCC 7120 asr1734 gene encodes a negative regulator of heterocyst development. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular microbiology

VOLUME: 64

Page Numbers: 782-94

Journal Abbreviation:

ISSN: 0950-382X

DAY: 3

MONTH: May

YEAR: 2007

The Anabaena sp. strain PCC 7120 asr1734 gene encodes a negative regulator of heterocyst development. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8712028

The Anabaena sp. strain PCC 7120 asr1734 gene encodes a negative regulator of heterocyst development. Keywords Mesh Terms:

KEYWORDS: Sulfur

MESH TERMS: metabolism

Chemical & Substance for Abstract: The Anabaena sp. strain PCC 7120 asr1734 gene encodes a negative regulator of heterocyst development. Information

Substance Name: Nitrogen

Registry Number: 7727-37-9

Grant and Affiliation Information for The Anabaena sp. strain PCC 7120 asr1734 gene encodes a negative regulator of heterocyst development.

AFFILIATION: Department of Biology, Texas A and M University, College Station, TX 77843-3258, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM36890

ACRONYM: GM

MEDLINETA: Mol Microbiol

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