The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis.

The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis. Abstract Text:

Joelle R Zavzavadjian,Sam Couture,Wei Sun Park,James Whalen,Stephen Lyon,Genie Lee,Eileen Fung,Qingli Mi,Jamie Liu,Estelle Wall,Leah Santat,Kavitha Dhandapani,Christine Kivork,Adrienne Driver,Xiaocui Zhu,Mi Sook Chang,Baljinder Randhawa,Elizabeth Gehrig,Heather Bryan,Mary Verghese,Andreia Maer,Brian Saunders,Yuhong Ning,Shankar Subramaniam,Tobias Meyer,Melvin I Simon,Nancy O'Rourke,Grischa Chandy,Iain D C Fraser,

Cellular responses to inputs that vary both temporally and spatially are determined by complex relationships between the components of cell signaling networks. Analysis of these relationships requires access to a wide range of experimental reagents and techniques, including the ability to express the protein components of the model cells in a variety of contexts. As part of the Alliance for Cellular Signaling, we developed a robust method for cloning large numbers of signaling ORFs into Gateway entry vectors, and we created a wide range of compatible expression platforms for proteomics applications. To date, we have generated over 3000 plasmids that are available to the scientific community via the American Type Culture Collection. We have established a website at www.signaling-gateway.org/data/plasmid/ that allows users to browse, search, and blast Alliance for Cellular Signaling plasmids. The collection primarily contains murine signaling ORFs with an emphasis on kinases and G protein signaling genes. Here we describe the cloning, databasing, and application of this proteomics resource for large scale subcellular localization screens in mammalian cell lines.

The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis. Publishing Authors By Initials

JR Zavzavadjian,S Couture,WS Park,J Whalen,S Lyon,G Lee,E Fung,Q Mi,J Liu,E Wall,L Santat,K Dhandapani,C Kivork,A Driver,X Zhu,MS Chang,B Randhawa,E Gehrig,H Bryan,M Verghese,A Maer,B Saunders,Y Ning,S Subramaniam,T Meyer,MI Simon,N O'Rourke,G Chandy,ID Fraser,

For similar biological phenomena, cell phenomena, and immunity: cell physiology: cell communication: signal transduction research abstracts see: biological phenomena, cell phenomena, and immunity: cell physiology: cell communication: signal transduction research

PUBMED ID PMID:

MEDLINE DATE:

The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular & cellular proteomics : MCP

VOLUME: 6

Page Numbers: 413-24

Journal Abbreviation: Mol. Cell Proteomics

ISSN: 1535-9476

DAY: 27

MONTH: 12

YEAR: 2006

The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101125647

The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis. Keywords Mesh Terms:

KEYWORDS: Signal Transduction

MESH TERMS: metabolism

Chemical & Substance for Abstract: The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis. Information

Substance Name: Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis.

AFFILIATION: Alliance for Cellular Signaling, California Institute of Technology, CA 94305, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: U54 GM062114

ACRONYM: GM

MEDLINETA: Mol Cell Proteomics

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis Related Publications

• Characterization of a spliced exon product of herpes simplex type-1 latency-associated transcript in productively infected cells.
• Circumstances of patient falls and injuries in 9 hospitals in a midwestern healthcare system.
• Comparing self-reported disease outcomes, diet, and lifestyles in a national cohort of black and white Seventh-day Adventists.
• Confocal quantification of cis-regulatory reporter gene expression in living sea urchin.
• Differences in protein mobility between pioneer versus follower growth cones.
• Effect of glutathione S-transferase polymorphisms and proximity to hazardous waste sites on time to systemic lupus erythematosus diagnosis: results from the Roxbury lupus project.
• Effects of fortified milk consumption on regional bone mineral accrual in Chinese girls.
• Effects of milk supplementation on cortical bone gain in Chinese girls aged 10-12 years.
• Epidemiology of methicillin-resistant Staphylococcus aureus colonization in a surgical intensive care unit.
• Estimating selection pressures from limited comparative data.
• Evaluation of Clostridium difficile-associated disease pressure as a risk factor for C difficile-associated disease.
• Factors influencing the effectiveness of research ethics committees.
• Feasibility of running clinics to collect biological specimens in a nationwide cohort study--Adventist Health Study-2.
• Genetic counselling in some common paediatric diseases.
• ICD-9 codes and surveillance for Clostridium difficile-associated disease.
• Impact of a methicillin-resistant Staphylococcus aureus active surveillance program on contact precaution utilization in a surgical intensive care unit.
• Injectable and macroporous calcium phosphate cement scaffold.
• Interplasmid transposition demonstrates piggyBac mobility in vertebrate species.
• Intra-articular glucocorticoid injection: an unusual cause of transient hypophosphataemia.
• Modulation of Abeta generation by small ubiquitin-like modifiers does not require conjugation to target proteins.
• Organizational research with impact: working backwards.
• Outcome of glenn anastomosis for heterotaxy syndrome with single ventricle.
• Patient safety event reporting in critical care: a study of three intensive care units.
• Prevalence of Clostridium difficile environmental contamination and strain variability in multiple health care facilities.
• Promoting science-based careers through student-directed learning.
• Severity of Clostridium difficile-associated disease (CDAD) in allogeneic stem cell transplant recipients: evaluation of a CDAD severity grading system.
• The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis.
• The impact of an antibiotic cycling program on empirical therapy for gram-negative infections.
• Transduction of brain by herpes simplex virus vectors.
• Translation research: where do we go from here?