The 15-lipoxygenase-1 expression may enhance the sensitivity to non-steroidal anti-inflammatory drug-induced apoptosis in colorectal cancers from patients who are treated with the compounds.

The 15-lipoxygenase-1 expression may enhance the sensitivity to non-steroidal anti-inflammatory drug-induced apoptosis in colorectal cancers from patients who are treated with the compounds. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The 15-lipoxygenase-1 expression may enhance the sensitivity to non-steroidal anti-inflammatory drug-induced apoptosis in colorectal cancers from patients who are treated with the compounds. Abstract Text:

Masahiro Yoshinaga,Hiroyuki Murao,Yosuke Kitamura,Koutaro Koga,Satoru Tsuruta,Hisato Igarashi,Kazuhiko Nakamura,Ryoichi Takayanagi,Masahiro Yoshinaga,Hiroyuki Murao,Yosuke Kitamura,Koutaro Koga,Satoru Tsuruta,Hisato Igarashi,Kazuhiko Nakamura,Ryoichi Takayanagi,Masahiro Yoshinaga,Hiroyuki Murao,Yosuke Kitamura,Koutaro Koga,Satoru Tsuruta,Hisato Igarashi,Kazuhiko Nakamura,Ryoichi Takayanagi,

Background and Aim: Non-steroidal anti-inflammatory drugs (NSAIDs) can prevent colorectal cancer (CRC), but their effect is limited. Recent studies have shown the involvement of 15-lipoxygenase-1 (15-LOX-1) in NSAID-induced apoptosis in colorectal carcinoma cells. We evaluate whether 15-LOX-1 expression influences the sensitivity of NSAID-induced apoptosis in CRCs. Methods: In 22 CRC surgical samples from NSAID users who had been constant for more than 5 years and 28 CRC surgical samples from NSAID non-users, the expressions of 15-LOX-1, cyclooxygenase-2 (COX-2), beta-catenin, and p53 were analyzed using immunohistochemistry. TUNEL assay was also performed for samples. The effects of the transient transfection of 15-LOX-1 cDNA on indomethacin-induced apoptosis were certified in HCT-116 cells. The effects of adding 13-S-hydroxyoctadecadinoic acid (13-S-HODE) on indomethacin-induced apoptosis were also examined in HCT-116 cells. The levels of apoptosis were determined by the analysis of the floating-cells ratio and DNA gel electrophoresis. Results: The expression of 15-LOX-1 on CRCs from NSAID users was significantly decreased compared with those from NSAID non-users; however, the expressions of other molecules were not significantly different between two groups. The levels of TUNEL scoring in samples from NSAID users were similar to those from NSAID non-users. Indomethacin (100 muM) induced less apoptosis in mocked cells, whereas the same concentrations of indomethacin enhanced the level of apoptosis in 15-LOX-1-transfected cells. 13-S-HODE also increased the level of indomethacin-induced apoptosis in cells. Conclusion: Results suggest that 15-LOX-1 expression may be one of the mechanisms which enhance the sensitivity to NSAID-induced apoptosis in CRCs from patients who are treated with the compounds.

The 15-lipoxygenase-1 expression may enhance the sensitivity to non-steroidal anti-inflammatory drug-induced apoptosis in colorectal cancers from patients who are treated with the compounds. Publishing Authors By Initials

M Yoshinaga,H Murao,Y Kitamura,K Koga,S Tsuruta,H Igarashi,K Nakamura,R Takayanagi,M Yoshinaga,H Murao,Y Kitamura,K Koga,S Tsuruta,H Igarashi,K Nakamura,R Takayanagi,M Yoshinaga,H Murao,Y Kitamura,K Koga,S Tsuruta,H Igarashi,K Nakamura,R Takayanagi,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

The 15-lipoxygenase-1 expression may enhance the sensitivity to non-steroidal anti-inflammatory drug-induced apoptosis in colorectal cancers from patients who are treated with the compounds. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of gastroenterology and hepatology

VOLUME: 22

Page Numbers: 2324-9

Journal Abbreviation: J. Gastroenterol. Hepatol.

ISSN: 0815-9319

DAY: 7

MONTH: 06

YEAR: 2007

The 15-lipoxygenase-1 expression may enhance the sensitivity to non-steroidal anti-inflammatory drug-induced apoptosis in colorectal cancers from patients who are treated with the compounds. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8607909

The 15-lipoxygenase-1 expression may enhance the sensitivity to non-steroidal anti-inflammatory drug-induced apoptosis in colorectal cancers from patients who are treated with the compounds. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: The 15-lipoxygenase-1 expression may enhance the sensitivity to non-steroidal anti-inflammatory drug-induced apoptosis in colorectal cancers from patients who are treated with the compounds. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The 15-lipoxygenase-1 expression may enhance the sensitivity to non-steroidal anti-inflammatory drug-induced apoptosis in colorectal cancers from patients who are treated with the compounds.

AFFILIATION: Department of Gastroenterology, National Hospital Organization Beppu Medical Center, Beppu, Japan.

Country: Australia

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Gastroenterol Hepatol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The 15-lipoxygenase-1 expression may enhance the sensitivity to non-steroidal anti-inflammatory drug-induced apoptosis in colorectal cancers from patients who are treated with the compounds Related Publications

• A Meckel's diverticulum with an ileal ulcer detected with double-balloon enteroscopy.
• A case of Zollinger-Ellison syndrome diagnosed three years after ulcer perforation in the third portion of the duodenum.
• A new parameter using serum lactate dehydrogenase and alanine aminotransferase level is useful for predicting the prognosis of patients at an early stage of acute liver injury: A retrospective study.
• Cathepsin K-dependent toll-like receptor 9 signaling revealed in experimental arthritis.
• Chemoradiotherapy with twice-weekly administration of low-dose gemcitabine for locally advanced pancreatic cancer.
• Clinical impact of endoscopic submucosal dissection for superficial adenocarcinoma located at the esophagogastric junction.
• Dietary patterns and A1C in Japanese men and women.
• Differential effect of sulfonylureas on production of reactive oxygen species and apoptosis in cultured pancreatic beta-cell line, MIN6.
• Histological studies of liver cirrhosis in white rats by thioacetamide feeding.
• Improvements of hypertriglyceridemia and hyperlacticemia in Japanese children with glycogen storage disease type Ia by medium-chain triglyceride milk.
• Lysophosphatidic acid induced nuclear translocation of nuclear factor-kappaB in Panc-1 cells by mobilizing cytosolic free calcium.
• Novel signaling pathways and therapeutic targets in osteoclasts.
• Optimal cut points of waist circumference for the clinical diagnosis of metabolic syndrome in the Japanese population.
• Oxytocin and appetite.
• Pitavastatin enhanced BMP-2 and osteocalcin expression by inhibition of Rho-associated kinase in human osteoblasts.
• Prospective comparative study on the acceptability of unsedated transnasal endoscopy in younger versus older patients.
• Stat1 functions as a cytoplasmic attenuator of Runx2 in the transcriptional program of osteoblast differentiation.
• The 15-lipoxygenase-1 expression may enhance the sensitivity to non-steroidal anti-inflammatory drug-induced apoptosis in colorectal cancers from patients who are treated with the compounds.
• The dynamic interplay between osteoclasts and the immune system.
• The lack of the C-terminal domain of adipose triglyceride lipase causes neutral lipid storage disease through impaired interactions with lipid droplets.
• The role of NFAT in osteoclast formation.
• Variation in parasystolic cycle length.
• [Frequency of gastroesophageal reflux disease (GERD) as a complication in patients with chronic liver diseases: estimation of frequency scale for the system of GERD]
• [Graves' disease with splenomegaly and pancytopenia, mimicking B-cell lymphoproliferative disease]
• [Guidelines on the management and treatment of glucocorticoid-induced osteoporosis (2004) in Japan]
• [Successful cyclosporine treatment for thrombocytopenia after salvage therapy with arsenic trioxide therapy followed by autologous hematopoietic stem cell transplantation in acute promyelocytic leukemia]
• [The role of oxidative stress in diabetic vascular complications]
• [Treatment of Osteoporosis and Evidence-Based Medicine. The effect of anabolic steroids on osteoporosis.]
• [Water and electrolyte metabolism during extracorporeal circulation]