Test for detection of disease-associated prion aggregate in the blood of infected but asymptomatic animals.

Test for detection of disease-associated prion aggregate in the blood of infected but asymptomatic animals. Research Abstract Details 

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Test for detection of disease-associated prion aggregate in the blood of infected but asymptomatic animals. Abstract Text:

Binggong Chang,Xin Cheng,Shaoman Yin,Tao Pan,Hongtao Zhang,Poki Wong,Shin-Chung Kang,Fan Xiao,Huimin Yan,Chaoyang Li,Lisa L Wolfe,Michael W Miller,Thomas Wisniewski,Mark I Greene,Man-Sun Sy,

We have developed a sensitive in vitro assay for detecting disease-associated prion aggregates by combining an aggregation-specific enzyme-linked immunosorbent assay (AS-ELISA) with the fluorescent amplification catalyzed by T7 RNA polymerase technique (FACTT). The new assay, named aggregation-specific FACTT (AS-FACTT), is much more sensitive than AS-ELISA and could detect prion aggregates in the brain of mice as early as 7 days after an intraperitoneal inoculation of PrP(Sc). However, AS-FACTT was still unable to detect prion aggregates in blood of infected mice. To further improve the detection limit of AS-FACTT, we added an additional prion amplification step (Am) and developed a third-generation assay, termed Am-A-FACTT. Am-A-FACTT has 100% sensitivity and specificity in detecting disease-associated prion aggregates in blood of infected mice at late but still asymptomatic stages of disease. At a very early stage, Am-A-FACTT had a sensitivity of 50% and a specificity of 100%. Most importantly, Am-A-FACTT also detects prion aggregates in blood of mule deer infected with the agent causing a naturally occurring prion disease, chronic wasting disease. Application of this assay to cattle, sheep, and humans could safeguard food supplies and prevent human contagion.

Test for detection of disease-associated prion aggregate in the blood of infected but asymptomatic animals. Publishing Authors By Initials

B Chang,X Cheng,S Yin,T Pan,H Zhang,P Wong,SC Kang,F Xiao,H Yan,C Li,LL Wolfe,MW Miller,T Wisniewski,MI Greene,MS Sy,

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Test for detection of disease-associated prion aggregate in the blood of infected but asymptomatic animals. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Clinical and vaccine immunology : CVI

VOLUME: 14

Page Numbers: 36-43

Journal Abbreviation: Clin. Vaccine Immunol.

ISSN: 1556-6811

DAY: 1

MONTH: 11

YEAR: 2006

Test for detection of disease-associated prion aggregate in the blood of infected but asymptomatic animals. Information

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LANGUAGE: eng

NlmUniqueID: 101252125

Test for detection of disease-associated prion aggregate in the blood of infected but asymptomatic animals. Keywords Mesh Terms:

KEYWORDS: Prions

MESH TERMS: immunology

Chemical & Substance for Abstract: Test for detection of disease-associated prion aggregate in the blood of infected but asymptomatic animals. Information

Substance Name: Prions

Registry Number: 0

Grant and Affiliation Information for Test for detection of disease-associated prion aggregate in the blood of infected but asymptomatic animals.

AFFILIATION: Institute of Pathology School of Medicine, Case Western Reserve University, Cleveland, Ohio 44107-1712, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS-045981-02

ACRONYM: NS

MEDLINETA: Clin Vaccine Immunol

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