Tertiary structure of mouse epidermal growth factor determined by two-dimensional 1H NMR.

Tertiary structure of mouse epidermal growth factor determined by two-dimensional 1H NMR. Research Abstract Details 

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Tertiary structure of mouse epidermal growth factor determined by two-dimensional 1H NMR. Abstract Text:

D Kohda,N Go,K Hayashi,F Inagaki,

The tertiary structure of mouse epidermal growth factor (EGF) in solution (28 degrees C, pH 2.0) was studied by two-dimensional NMR spectroscopy. Proton-proton distance constraints derived from NOESY spectra were used to construct a mechanical molecular model of mouse EGF, which was subsequently checked by means of a preliminary distance geometry calculation. The chain-folds in the two structural domains of mouse EGF were very similar to those previously reported (Montelione et al. (1987) Proc. Natl. Acad. Sci. U.S. 84, 5226-5230). However, the relative orientations of the two domains were different. Because we could assign much more inter-domain NOEs, the relative orientations of the two domains were well determined in our model. The hollow between the two domains may function as a binding site for the EGF receptor.

Tertiary structure of mouse epidermal growth factor determined by two-dimensional 1H NMR. Publishing Authors By Initials

D Kohda,N Go,K Hayashi,F Inagaki,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: molecular conformation: protein conformation research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: molecular conformation: protein conformation research

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Tertiary structure of mouse epidermal growth factor determined by two-dimensional 1H NMR. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 103

Page Numbers: 741-3

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1988

Tertiary structure of mouse epidermal growth factor determined by two-dimensional 1H NMR. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Tertiary structure of mouse epidermal growth factor determined by two-dimensional 1H NMR. Keywords Mesh Terms:

KEYWORDS: Protein Conformation

MESH TERMS: methods

Chemical & Substance for Abstract: Tertiary structure of mouse epidermal growth factor determined by two-dimensional 1H NMR. Information

Substance Name: Epidermal Growth Factor

Registry Number: 62229-50-9

Grant and Affiliation Information for Tertiary structure of mouse epidermal growth factor determined by two-dimensional 1H NMR.

AFFILIATION: Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science.

Country: JAPAN

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MEDLINETA: J Biochem

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