Ternary complex formation on the adenovirus packaging sequence by the IVa2 and L4 22-kilodalton proteins.

Ternary complex formation on the adenovirus packaging sequence by the IVa2 and L4 22-kilodalton proteins. Research Abstract Details 

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Ternary complex formation on the adenovirus packaging sequence by the IVa2 and L4 22-kilodalton proteins. Abstract Text:

Sean G Ewing,Serena A Byrd,Joan B Christensen,Ryan E Tyler,Michael J Imperiale,Sean G Ewing,Serena A Byrd,Joan B Christensen,Ryan E Tyler,Michael J Imperiale,

Assembly of infectious adenovirus particles requires seven functionally redundant elements at the left end of the genome, termed A repeats, that direct packaging of the DNA. Previous studies revealed that the viral IVa2 protein alone interacts with specific sequences in the A repeats but that additional IVa2-containing complexes observed during infection require the viral L4 22-kDa protein. In this report, we purified a recombinant form of the 22-kDa protein to characterize its DNA binding properties. In electrophoretic mobility shift assay analyses, the 22-kDa protein alone did not interact with the A repeats but it did form complexes on them in the presence of the IVa2 protein. These complexes were identical to those seen in extracts from infected cells and had the same DNA sequence dependence. Furthermore, we provide data that the 22-kDa protein enhances binding of the IVa2 protein to the A repeats and that multiple binding sites in the packaging sequence augment this activity. These data support a cooperative role of the IVa2 and 22-kDa proteins in packaging and assembly.

Ternary complex formation on the adenovirus packaging sequence by the IVa2 and L4 22-kilodalton proteins. Publishing Authors By Initials

SG Ewing,SA Byrd,JB Christensen,RE Tyler,MJ Imperiale,SG Ewing,SA Byrd,JB Christensen,RE Tyler,MJ Imperiale,

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Ternary complex formation on the adenovirus packaging sequence by the IVa2 and L4 22-kilodalton proteins. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of virology

VOLUME: 81

Page Numbers: 12450-7

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 5

MONTH: 09

YEAR: 2007

Ternary complex formation on the adenovirus packaging sequence by the IVa2 and L4 22-kilodalton proteins. Information

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LANGUAGE: eng

NlmUniqueID: 113724

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Grant and Affiliation Information for Ternary complex formation on the adenovirus packaging sequence by the IVa2 and L4 22-kilodalton proteins.

AFFILIATION: Department of Microbiology and Immunology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Country: United States

AGENCY: United States NCI

GRANT: P30 CA46592

ACRONYM: CA

MEDLINETA: J Virol

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