Temporally distinctive changes of alternative splicing patterns during myogenic differentiation of C2C12 cells.

Temporally distinctive changes of alternative splicing patterns during myogenic differentiation of C2C12 cells. Research Abstract Details 

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Temporally distinctive changes of alternative splicing patterns during myogenic differentiation of C2C12 cells. Abstract Text:

Y Harada,M Nakamura,A Asano,

It is well known that skeletal muscle differentiation is accompanied by the appearance of many muscle-specific components and that some of these components are generated through muscle-specific alternative splicing. It is not clear, however, in what manner, including timing, the system that regulates the muscle-specific splicing reactions is constructed during the process of myogenic differentiation. We simultaneously examined the changes in several splicing patterns for the neural cell adhesion molecule (NCAM), beta-tropomyosin, and M-type pyruvate kinase genes during myogenic differentiation of cultured myoblasts using the reverse transcription-polymerase chain reaction method. The NCAM glycosylphosphatidylinositol anchor form increased in preference to the transmembrane form immediately after the induction of differentiation, while the selection of NCAM MSD1 (muscle-specific domain 1) exons started and abruptly increased at about the time when cell-fusion appeared. M2-type pyruvate kinase was gradually substituted for the M1-type molecule. Skeletal muscle-type beta-tropomyosin was predominantly selected even in myoblasts in the growth medium. As a result, each transcript of these genes independently showed a temporally distinctive pattern of change in isoform selecting during the myogenic differentiation of C2C12 cells. These observations suggest that some independent regulation of alternative splicing reactions should occur during myogenic differentiation.

Temporally distinctive changes of alternative splicing patterns during myogenic differentiation of C2C12 cells. Publishing Authors By Initials

Y Harada,M Nakamura,A Asano,

For similar macromolecular substances: polymers: biopolymers: microfilament proteins: tropomyosin research abstracts see: macromolecular substances: polymers: biopolymers: microfilament proteins: tropomyosin research

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Temporally distinctive changes of alternative splicing patterns during myogenic differentiation of C2C12 cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 118

Page Numbers: 780-90

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1995

Temporally distinctive changes of alternative splicing patterns during myogenic differentiation of C2C12 cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Temporally distinctive changes of alternative splicing patterns during myogenic differentiation of C2C12 cells. Keywords Mesh Terms:

KEYWORDS: Tropomyosin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Temporally distinctive changes of alternative splicing patterns during myogenic differentiation of C2C12 cells. Information

Substance Name: Pyruvate Kinase

Registry Number: EC 2.7.1.40

Grant and Affiliation Information for Temporally distinctive changes of alternative splicing patterns during myogenic differentiation of C2C12 cells.

AFFILIATION: Institute for Protein Research, Osaka University.

Country: JAPAN

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MEDLINETA: J Biochem

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