Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture.

Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture. Research Abstract Details 

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Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture. Abstract Text:

Mainak Das,Neelima Bhargava,Abhijeet Bhalkikar,Jung Fong Kang,James J Hickman,Mainak Das,Neelima Bhargava,Abhijeet Bhalkikar,Jung Fong Kang,James J Hickman,

The ability to culture functional adult mammalian spinal cord neurons represents an important step in the understanding and treatment of a spectrum of neurological disorders including spinal cord injury. Previously, the limited functional recovery of these cells, as characterized by a diminished ability to initiate action potentials and to exhibit repetitive firing patterns, has arisen as a major impediment to their physiological relevance. In this report, we demonstrate that single temporal doses of the neurotransmitters serotonin, glutamate (N-acetyl-dl-glutamic acid) and acetylcholine-chloride lead to the full electrophysiological functional recovery of adult mammalian spinal cord neurons, when they are cultured under defined serum-free conditions. Approximately 60% of the neurons treated regained their electrophysiological signature, often firing single, double and, most importantly, multiple action potentials.

Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture. Publishing Authors By Initials

M Das,N Bhargava,A Bhalkikar,JF Kang,JJ Hickman,M Das,N Bhargava,A Bhalkikar,JF Kang,JJ Hickman,

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Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Experimental neurology

VOLUME: 209

Page Numbers: 171-80

Journal Abbreviation: Exp. Neurol.

ISSN: 0014-4886

DAY: 26

MONTH: 09

YEAR: 2007

Temporal neurotransmitter conditioning restores the functional activity of adult spinal cord neurons in long-term culture. Information

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LANGUAGE: eng

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AFFILIATION: NanoScience Technology Center, 12424 Research Parkway, Suite 400, University of Central Florida, Orlando, FL 32826, USA.

Country: United States

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MEDLINETA: Exp Neurol

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