Template misalignment in multisubunit RNA polymerases and transcription fidelity.

Template misalignment in multisubunit RNA polymerases and transcription fidelity. Research Abstract Details 

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Template misalignment in multisubunit RNA polymerases and transcription fidelity. Abstract Text:

Ekaterina Kashkina,Michael Anikin,Florian Brueckner,Richard T Pomerantz,William T McAllister,Patrick Cramer,Dmitry Temiakov,

Recent work showed that the single-subunit T7 RNA polymerase (RNAP) can generate misincorporation errors by a mechanism that involves misalignment of the DNA template strand. Here, we show that the same mechanism can produce errors during transcription by the multisubunit yeast RNAP II and bacterial RNAPs. Fluorescence spectroscopy reveals a reorganization of the template strand during this process, and molecular modeling suggests an open space above the polymerase active site that could accommodate a misaligned base. Substrate competition assays indicate that template misalignment, not misincorporation, is the preferred mechanism for substitution errors by cellular RNAPs. Misalignment could account for data previously taken as evidence for additional NTP binding sites downstream of the active site. Analysis of the effects of different template topologies on misincorporation indicates that the duplex DNA immediately downstream of the active site plays an important role in transcription fidelity.

Template misalignment in multisubunit RNA polymerases and transcription fidelity. Publishing Authors By Initials

E Kashkina,M Anikin,F Brueckner,RT Pomerantz,WT McAllister,P Cramer,D Temiakov,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Template misalignment in multisubunit RNA polymerases and transcription fidelity. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular cell

VOLUME: 24

Page Numbers: 257-66

Journal Abbreviation: Mol. Cell

ISSN: 1097-2765

DAY: 20

MONTH: Oct

YEAR: 2006

Template misalignment in multisubunit RNA polymerases and transcription fidelity. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9802571

Template misalignment in multisubunit RNA polymerases and transcription fidelity. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Template misalignment in multisubunit RNA polymerases and transcription fidelity. Information

Substance Name: DNA-Directed RNA Polymerases

Registry Number: EC 2.7.7.6

Grant and Affiliation Information for Template misalignment in multisubunit RNA polymerases and transcription fidelity.

AFFILIATION: Department of Cell Biology, School of Osteopathic Medicine, University of Medicine and Dentistry of New Jersey, 42 East Laurel Road, Stratford, New Jersey 08084, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM38147

ACRONYM: GM

MEDLINETA: Mol Cell

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