Temperature-induced regulation of RpoS by a small RNA in Borrelia burgdorferi.

Temperature-induced regulation of RpoS by a small RNA in Borrelia burgdorferi. Research Abstract Details 

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Temperature-induced regulation of RpoS by a small RNA in Borrelia burgdorferi. Abstract Text:

Meghan C Lybecker,D Scott Samuels,

The alternative sigma factor RpoS (sigma38 or sigmaS) plays a central role in the reciprocal regulation of the virulence-associated major outer surface proteins OspC and OspA in Borrelia burgdorferi, the Lyme disease spirochete. Temperature is one of the key environmental signals controlling RpoS, but the molecular mechanism by which the signal is transduced remains unknown. Herein, we identify and describe a small non-coding RNA, DsrABb, that regulates the temperature-induced increase in RpoS. A novel 5' end of the rpoS mRNA was identified and DsrABb has the potential to extensively base-pair with the upstream region of this rpoS transcript. We demonstrate that B. burgdorferi strains lacking DsrABb do not upregulate RpoS and OspC in response to an increase in temperature, but do regulate RpoS and OspC in response to changes in pH and cell density. Analyses of the rpoS and ospC steady-state mRNA levels in the dsrABb mutant indicate that DsrABb regulates RpoS post-transcriptionally. The 5' and 3' ends of DsrABb were mapped, demonstrating that at least four species exist with sizes ranging from 213 to 352 nucleotides. We hypothesize that DsrABb binds to the upstream region of the rpoS mRNA and stimulates translation by releasing the Shine-Dalgarno sequence and start site from a stable secondary structure. Therefore, we postulate that DsrABb is a molecular thermometer regulating RpoS in Borrelia burgdorferi.

Temperature-induced regulation of RpoS by a small RNA in Borrelia burgdorferi. Publishing Authors By Initials

MC Lybecker,DS Samuels,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

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Temperature-induced regulation of RpoS by a small RNA in Borrelia burgdorferi. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular microbiology

VOLUME: 64

Page Numbers: 1075-89

Journal Abbreviation: Mol. Microbiol.

ISSN: 0950-382X

DAY: 3

MONTH: May

YEAR: 2007

Temperature-induced regulation of RpoS by a small RNA in Borrelia burgdorferi. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8712028

Temperature-induced regulation of RpoS by a small RNA in Borrelia burgdorferi. Keywords Mesh Terms:

KEYWORDS: Temperature

MESH TERMS: genetics

Chemical & Substance for Abstract: Temperature-induced regulation of RpoS by a small RNA in Borrelia burgdorferi. Information

Substance Name: sigma factor KatF protein, Bacteria

Registry Number: 0

Grant and Affiliation Information for Temperature-induced regulation of RpoS by a small RNA in Borrelia burgdorferi.

AFFILIATION: Division of Biological Sciences, The University of Montana, Missoula, MT 59812-4824, USA.

Country: England

AGENCY: United States NIAID

GRANT: AI051486

ACRONYM: AI

MEDLINETA: Mol Microbiol

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