Technical note: measurement of ferritin in bovine milk and its clinical significance.

Technical note: measurement of ferritin in bovine milk and its clinical significance. Research Abstract Details 

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Technical note: measurement of ferritin in bovine milk and its clinical significance. Abstract Text:

K Orino,S Watanabe,H Ohtsuka,M Kohiruimaki,K Watanabe,

A quantitative ELISA was developed for bovine milk ferritin with an assay limit of 0.16 ng/mL of bovine spleen ferritin. Ferritin-binding activity was detected in bovine milk samples, and this binding activity was inhibited by increasing ionic strength with the addition of 0.5 M (NH4)2SO4. Heat treatment (60 degrees C, 20 min) of bovine milk in the presence of 0.5 M (NH4)2SO4 resulted in a 15 to 58% increase in ferritin concentrations compared with untreated samples. Although the recovery of bovine spleen ferritin added to milk was still low (55 to 90%), even in the presence of increased ionic strength with 0.5 M (NH4)2SO4, recovery was improved by heat treatment at 60 degrees C for 20 min (92 to 95%). Milk ferritin concentrations in 30 milk samples from quarters of 25 cows with mastitis (mean +/- SE: 134.2 +/- 28.7 ng/mL) were significantly higher than those in 17 quarter milk samples from 17 noninfected lactating cows (7.2 +/- 1.2 ng/mL), suggesting that bovine milk contains putative ferritin-binding proteins that inhibit immunoassay for milk ferritin and that bovine milk ferritin is an indicator of IMI.

Technical note: measurement of ferritin in bovine milk and its clinical significance. Publishing Authors By Initials

K Orino,S Watanabe,H Ohtsuka,M Kohiruimaki,K Watanabe,

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Technical note: measurement of ferritin in bovine milk and its clinical significance. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of dairy science

VOLUME: 89

Page Numbers: 3842-5

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ISSN: 1525-3198

DAY: 8

MONTH: Oct

YEAR: 2006

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LANGUAGE: eng

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AFFILIATION: Laboratories of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori 034-8628, Japan. orino@vmas.kitasato-u.ac.jp

Country: United States

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MEDLINETA: J Dairy Sci

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