Technetium-99m labeled monoclonal antibodies: evaluation of reducing agents.

Technetium-99m labeled monoclonal antibodies: evaluation of reducing agents. Research Abstract Details 

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Technetium-99m labeled monoclonal antibodies: evaluation of reducing agents. Abstract Text:

M L Thakur,J DeFulvio,M D Richard,C H Park,

We have evaluated five compounds, stannous chloride (SnCl2), 2-mercaptoethanol (2-ME), dithiothreitol (DTT), dithioerythritol (DTE), and ascorbic acid (AA) to reduce monoclonal antibody MoAb (disulfide groups and compared their efficacy for labeling MoAbs with 99mTc. The reduction of 99mTc with dithionite at pH 11 was nearly quantitative. The use of AA, at a molar ratio of 3500:1, for three IgG and three IgM antibodies examined, gave a labeling efficiency greater than 95%. Hence no purification was needed. The immunospecificity of AA preparations determined by specific antigen assay was 84 +/- 1% for an IgM and 82.6 +/- 1.1% for an IgG, highest among all agents tested. The stability of the tracer was evaluated by challenging the product with such 99mTc avid agents as cysteine, DTPA, and human serum albumin. By HPLC analysis, no 99mTc was transchelated using chelating agent to protein molar ratios as high as 500:1. In two separate groups of five mice each, the liver uptake at 4 h post injection averaged 6.8 +/- 2.9% per gram for 125I-TNT-1 (IgG) and 6 +/- 5.1% per gram for the same MoAb labeled with 99mTc using AA. The AA technique promises to label antibodies with 99mTc and perhaps with 186Re, by a simple "kit" procedure.

Technetium-99m labeled monoclonal antibodies: evaluation of reducing agents. Publishing Authors By Initials

ML Thakur,J DeFulvio,MD Richard,CH Park,

For similar inorganic chemicals: tin compounds research abstracts see: inorganic chemicals: tin compounds research

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Technetium-99m labeled monoclonal antibodies: evaluation of reducing agents. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: International journal of radiation applications an

VOLUME: 18

Page Numbers: 227-33

Journal Abbreviation:

ISSN: 0883-2897

DAY: 21

MONTH: 02

YEAR: 1991

Technetium-99m labeled monoclonal antibodies: evaluation of reducing agents. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8611098

Technetium-99m labeled monoclonal antibodies: evaluation of reducing agents. Keywords Mesh Terms:

KEYWORDS: Tin Compounds

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Chemical & Substance for Abstract: Technetium-99m labeled monoclonal antibodies: evaluation of reducing agents. Information

Substance Name: stannous chloride

Registry Number: 7772-99-8

Grant and Affiliation Information for Technetium-99m labeled monoclonal antibodies: evaluation of reducing agents.

AFFILIATION: Department of Radiation Oncology and Nuclear Medicine, Thomas Jefferson University, Philadelphia, PA 19107.

Country: UNITED STATES

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MEDLINETA: Int J Rad Appl Instrum B

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