Teaching resources. A biochemical assay for acetylcholinesterase activity in PC12 cells.

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Teaching resources. A biochemical assay for acetylcholinesterase activity in PC12 cells. Abstract Text:

Paul J Schwartz,Jay A Blundon,Elizabeth M Adler,

This lab describes two biochemical assays: One for measuring acetylcholinesterase activity and one for measuring protein concentration. Students learn how to manipulate small-volume samples, use a standard spectrophotometer or a microplate reader spectrophotometer, construct a standard curve, and normalize data. The lab is intended to be used in conjunction with a cell culture lab in which PC12 cells are exposed to various agents that influence their phenotypic state.

Teaching resources. A biochemical assay for acetylcholinesterase activity in PC12 cells. Publishing Authors By Initials

PJ Schwartz,JA Blundon,EM Adler,

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Teaching resources. A biochemical assay for acetylcholinesterase activity in PC12 cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Science's STKE : signal transduction knowledge env

VOLUME: 2007

Page Numbers: tr2

Journal Abbreviation: Sci. STKE

ISSN: 1525-8882

DAY: 28

MONTH: Jul

YEAR: 2007

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LANGUAGE: eng

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Grant and Affiliation Information for Teaching resources. A biochemical assay for acetylcholinesterase activity in PC12 cells.

AFFILIATION: Department of Neurological Surgery, University of Medicine and Dentistry of New Jersey, Newark, NJ 07101, USA.

Country: United States

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