Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry.

Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry. Abstract Text:

Caterina Pettinella,Seon Hwa Lee,Francesco Cipollone,Ian A Blair,

The quantitative analysis of fatty acid composition in atherosclerotic plaques provides a way to monitor the underlying etiology of atherosclerosis. Previously, the method of choice for analyzing fatty acids in biological samples was gas chromatography/mass spectrometry (GC/MS); however, recent developments in electrospray ionization (ESI)/liquid chromatography (LC)/tandem mass spectrometry have made it a superior alternative. Previous research has largely focused on global analyses of intact lipids rather than more targeted analysis of the fatty acids themselves. We have now developed a targeted, stable isotope dilution LC-electrospray ionization/multiple reaction monitoring/MS method for the quantitative analysis of 10 fatty acids (myristic, palmitic, stearic, oleic, linoleic, alpha-linolenic, gamma-linolenic, arachidonic, eicosapentaenoic, and docosahexaenoic acids) using their trimethylaminoethyl ester (TMAE) derivatives to improve sensitivity. The method was validated, had a detection limit in the fmol range, and was used in the analysis of fatty acids in atherosclerotic plaques from carotid arteries.

Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry. Publishing Authors By Initials

C Pettinella,SH Lee,F Cipollone,IA Blair,

For similar investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research abstracts see: investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research

PUBMED ID PMID:

MEDLINE DATE:

Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry. Journal Published:

PUBLICATION TYPE: Validation Studies

Journal: Journal of chromatography. B, Analytical technolog

VOLUME: 850

Page Numbers: 168-76

Journal Abbreviation:

ISSN: 1570-0232

DAY: 14

MONTH: 12

YEAR: 2006

Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101139554

Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry. Keywords Mesh Terms:

KEYWORDS: Tandem Mass Spectrometry

MESH TERMS: methods

Chemical & Substance for Abstract: Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry. Information

Substance Name: Fatty Acids

Registry Number: 0

Grant and Affiliation Information for Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry.

AFFILIATION: Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, 854 BRB II/III, 421 Curie Boulevard, Philadelphia, PA 19104-6160, USA.

Country: Netherlands

AGENCY: United States NCI

GRANT: R01CA91016

ACRONYM: CA

MEDLINETA: J Chromatogr B Analyt Technol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry Related Publications

• 706.
• Accurate and efficient detection of pulmonary seed embolization in prostate iodine-125 permanent brachytherapy with a collimated gamma scintillation survey meter.
• An innovative, effective and cost effective survey method using a survey-check response format.
• Analysis of phosphorylation sites on focal adhesion kinase using nanospray liquid chromatography/multiple reaction monitoring mass spectrometry.
• Characterization of an etoposide-glutathione conjugate derived from metabolic activation by human cytochrome p450.
• Classifying Weight Status Using Body Mass Index and Percent Body Fat: 2130: Board #43 June 1 9:00 AM - 10:30 AM.
• Construct validity evidence for single-response items to estimate physical activity levels in large sample studies.
• Convenient and efficient syntheses of 4-hydroxy-2(E)-nonenal and 4-oxo-2(E)-nonenal.
• Coronary artery calcium, exercise tolerance, and CHD events in asymptomatic men.
• Death receptor-3 mediates apoptosis in human osteoblasts under narrowly regulated conditions.
• Does Faculty Practice Validate the NP Educator Role?
• Effect of a dose-response exercise study on daily step activity in postmenopausal women: 1558: board #48 may 30 3:30 PM - 5:00 PM.
• Effects of different doses of physical activity on cardiorespiratory fitness among sedentary, overweight or obese postmenopausal women with elevated blood pressure: a randomized controlled trial.
• Endogenous glutathione adducts.
• Estrogen receptor-beta modulates synthesis of bone matrix proteins in human osteoblast-like MG63 cells.
• Graded Doses of Exercise and Insulin Resistance in Nondiabetic Post-Menopausal Women: The DREW Study: 1540: Board #30 May 30 2:00 PM - 3:30 PM.
• Heightened sensitivity to facial expressions of emotion in borderline personality disorder.
• LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells.
• Ml01 competence reviews in new zealand and the aftermath.
• Modulation of emotion by cognition and cognition by emotion.
• Mutational analysis of the flagellar protein FliG: sites of interaction with FliM and implications for organization of the switch complex.
• Occupation and breast cancer risk in Polish women: a population-based case-control study.
• Proteomics-based strategy to identify biomarkers and pharmacological targets in leukemias with t(4;11) translocations.
• Quantification of intracellular homocysteine by stable isotope dilution liquid chromatography/tandem mass spectrometry.
• Quantitative analysis of amyloid beta peptides in cerebrospinal fluid of Alzheimer's disease patients by immunoaffinity purification and stable isotope dilution liquid chromatography/negative electrospray ionization tandem mass spectrometry.
• Response options and expectations of reward in decision-making: the differential roles of dorsal and rostral anterior cingulate cortex.
• Subgroup Interactions and Changes in Cardiorespiratory Fitness among Postmenopausal Women: The DREW Study: 1556: Board #46 May 30 3:30 PM - 5:00 PM.
• Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry.
• The moving emission features in SS433 require a dynamical interpretation.
• [Anesthesia arrived unexpectedly and quickly; the anesthesiologist appeared more slowly. The story of the dissemination of the discovery of anesthesia in the United Kingdom and the United States of America.]