Targeted expression of csCSF-1 in op/op mice ameliorates tooth defects.

Targeted expression of csCSF-1 in op/op mice ameliorates tooth defects. Research Abstract Details 

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Targeted expression of csCSF-1 in op/op mice ameliorates tooth defects. Abstract Text:

S Abboud Werner,J Gluhak-Heinrich,K Woodruff,Y Wittrant,L Cardenas,M Roudier,M MacDougall,S Abboud Werner,J Gluhak-Heinrich,K Woodruff,Y Wittrant,L Cardenas,M Roudier,M MacDougall,

OBJECTIVE: The aim of this study was to characterize the tooth phenotype of CSF-1-deficient op/op mice and determine whether expression of csCSF-1 in these mice has a role in primary tooth matrix formation. DESIGN: Ameloblasts and odontoblasts, isolated from wt/wt frozen sections using laser capture microdissection, were analysed for csCSF-1, sCSF-1 and CSF-1R mRNA by RT-PCR. Mandibles, excised from 8 days op/op and wt/wt littermates, were examined for tooth morphology as well as amelogenin and DMP1 expression using in situ hybridisation. op/opCS transgenic mice, expressing csCSF-1 in teeth and bone using the osteocalcin promoter, were generated. Skeletal X-rays and histomorphometry were performed; teeth were analysed for morphology and matrix proteins. RESULTS: Normal dental cells in vivo express both CSF-1 isoforms and CSF-1R. Compared to wt/wt, op/op teeth prior to eruption showed altered dental cell morphology and dramatic reduction in DMP1 transcripts. op/opCS mice showed marked resolution of osteopetrosis, tooth eruption and teeth that resembled amelogenesis imperfecta-like phenotype. At 3 weeks, op/op teeth showed severe enamel and dentin defects and barely detectable amelogenin and DMP1. In op/opCS mice, DMP1 in odontoblasts increased to near normal and dentin morphology was restored; amelogenin also increased. Enamel integrity improved in op/opCS, although it was thinner than wt enamel. CONCLUSIONS: Results demonstrate that ameloblasts and odontoblasts are a source and potential target of CSF-1 isoforms in vivo. Expression of csCSF-1 within the tooth microenvironment is essential for normal tooth morphogenesis and may provide a mechanism for coordinating the process of tooth eruption with endogenous matrix formation.

Targeted expression of csCSF-1 in op/op mice ameliorates tooth defects. Publishing Authors By Initials

SA Werner,J Gluhak-Heinrich,K Woodruff,Y Wittrant,L Cardenas,M Roudier,M MacDougall,SA Werner,J Gluhak-Heinrich,K Woodruff,Y Wittrant,L Cardenas,M Roudier,M MacDougall,

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Targeted expression of csCSF-1 in op/op mice ameliorates tooth defects. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Archives of oral biology

VOLUME: 52

Page Numbers: 432-43

Journal Abbreviation: Arch. Oral Biol.

ISSN: 0003-9969

DAY: 28

MONTH: 11

YEAR: 2006

Targeted expression of csCSF-1 in op/op mice ameliorates tooth defects. Information

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LANGUAGE: eng

NlmUniqueID: 116711

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AFFILIATION: Department of Pathology, University of Texas Health Science Center, 7703 Floyd Curl Drive and South Texas Veteran's Health Care System, Audi L. Murphy Division, San Antonio, TX 78229, USA. abboudwerner@uthscsa.edu

Country: England

AGENCY: United States NIDCR

GRANT: R01 DE015857-03

ACRONYM: DE

MEDLINETA: Arch Oral Biol

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