Target DNA structure plays a critical role in RAG transposition.

Target DNA structure plays a critical role in RAG transposition. Research Abstract Details 

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Target DNA structure plays a critical role in RAG transposition. Abstract Text:

Jennifer E Posey,Malgorzata J Pytlos,Richard R Sinden,David B Roth,

Antigen receptor gene rearrangements are initiated by the RAG1/2 protein complex, which recognizes specific DNA sequences termed RSS (recombination signal sequences). The RAG recombinase can also catalyze transposition: integration of a DNA segment bounded by RSS into an unrelated DNA target. For reasons that remain poorly understood, such events occur readily in vitro, but are rarely detected in vivo. Previous work showed that non-B DNA structures, particularly hairpins, stimulate transposition. Here we show that the sequence of the four nucleotides at a hairpin tip modulates transposition efficiency over a surprisingly wide (>100-fold) range. Some hairpin targets stimulate extraordinarily efficient transposition (up to 15%); one serves as a potent and specific transposition inhibitor, blocking capture of targets and destabilizing preformed target capture complexes. These findings suggest novel regulatory possibilities and may provide insight into the activities of other transposases.

Target DNA structure plays a critical role in RAG transposition. Publishing Authors By Initials

JE Posey,MJ Pytlos,RR Sinden,DB Roth,

For similar enzymes and coenzymes: enzymes: recombinases: integrases: transposases research abstracts see: enzymes and coenzymes: enzymes: recombinases: integrases: transposases research

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Target DNA structure plays a critical role in RAG transposition. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: PLoS biology

VOLUME: 4

Page Numbers: e350

Journal Abbreviation: PLoS Biol.

ISSN: 1545-7885

DAY: 3

MONTH: Nov

YEAR: 2006

Target DNA structure plays a critical role in RAG transposition. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101183755

Target DNA structure plays a critical role in RAG transposition. Keywords Mesh Terms:

KEYWORDS: Transposases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Target DNA structure plays a critical role in RAG transposition. Information

Substance Name: Transposases

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Target DNA structure plays a critical role in RAG transposition.

AFFILIATION: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America.

Country: United States

AGENCY: United States NIEHS

GRANT: ES05508

ACRONYM: ES

MEDLINETA: PLoS Biol

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