T cells recognize PD(N/T)R motif common in a variable number of tandem repeat and degenerate repeat sequences of MUC1.

T cells recognize PD(N/T)R motif common in a variable number of tandem repeat and degenerate repeat sequences of MUC1. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

T cells recognize PD(N/T)R motif common in a variable number of tandem repeat and degenerate repeat sequences of MUC1. Abstract Text:

Vladimir M Pisarev,Leo Kinarsky,Thomas Caffrey,Franz-George Hanisch,Sam Sanderson,Michael A Hollingsworth,Simon Sherman,

The tumor-associated antigen MUC1 is a transmembrane glycoprotein, which is overexpressed in human carcinomas. Peptide epitopes, containing the PDTR fragment from the variable number of tandem repeat (VNTR) domains of MUC1 have been found to be immunodominant in T-cell and B-cell responses. However, little is known about the immunogenicity and specificity of T-cell epitopes from other regions of MUC1 that may also participate in immune responses against tumors. In this study, the combination of immunoinformatics, molecular modeling and a vaccine adjuvant strategy were used to predict and describe a novel T-cell epitope, SAPDNRPAL, located within the degenerate tandem repeat of MUC1. This peptide possesses structural similarity to both VNTR-derived SAPDTRPAP and Sendai virus peptide FAPGNYPAL, which are known to induce cytotoxic T lymphocytes (CTL). We found that SAPDNRPAL had a higher affinity for mouse H-D(b), H-2K(b) and human HLA-A2 molecules than SAPDTRPAP. A chimeric peptide (CP) containing SAPDNRPAL and an adjuvant C5a-derived decapeptide induced epitope-specific type 1 T cells in human MUC1 transgenic mice (ELISPOT). Mice that received dendritic cells (DC) pulsed with the CP or a 25-mer peptide containing the SAPDNRPAL sequence showed increased frequencies of SAPDNRPAL- and SAPDTRPAP-specific interferon-gamma producing T cells. PDTR-specific antibody 214D4 reacted with both SAPDNRPAL and SAPDTRPAP (ELISA). Altogether, our data suggest that the degenerate MUC1 repeat sequence contains the immunogenic T-cell epitope SAPDNRPAL, which is cross-reactive with the VNTR-derived peptide SAPDTRPAP. We suggest that the use of immunogenic PDNR-containing epitope(s) in vaccine strategies could be beneficial for developing increased, PD(N/T)R motif-specific T-cell responses against tumors expressing MUC1.

T cells recognize PD(N/T)R motif common in a variable number of tandem repeat and degenerate repeat sequences of MUC1. Publishing Authors By Initials

VM Pisarev,L Kinarsky,T Caffrey,FG Hanisch,S Sanderson,MA Hollingsworth,S Sherman,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: base sequence: repetitive sequences, nucleic acid: tandem repeat sequences research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: base sequence: repetitive sequences, nucleic acid: tandem repeat sequences research

PUBMED ID PMID:

MEDLINE DATE:

T cells recognize PD(N/T)R motif common in a variable number of tandem repeat and degenerate repeat sequences of MUC1. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: International immunopharmacology

VOLUME: 5

Page Numbers: 315-30

Journal Abbreviation: Int. Immunopharmacol.

ISSN: 1567-5769

DAY: 1

MONTH: Feb

YEAR: 2005

T cells recognize PD(N/T)R motif common in a variable number of tandem repeat and degenerate repeat sequences of MUC1. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100965259

T cells recognize PD(N/T)R motif common in a variable number of tandem repeat and degenerate repeat sequences of MUC1. Keywords Mesh Terms:

KEYWORDS: Tandem Repeat Sequences

MESH TERMS: immunology

Chemical & Substance for Abstract: T cells recognize PD(N/T)R motif common in a variable number of tandem repeat and degenerate repeat sequences of MUC1. Information

Substance Name: Peptide Fragments

Registry Number: 0

Grant and Affiliation Information for T cells recognize PD(N/T)R motif common in a variable number of tandem repeat and degenerate repeat sequences of MUC1.

AFFILIATION: Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198-6805, USA.

Country: Netherlands

AGENCY: United States NCI

GRANT: 5R01CA84106

ACRONYM: CA

MEDLINETA: Int Immunopharmacol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

T cells recognize PDN/TR motif common in a variable number of tandem repeat and degenerate repeat sequences of MUC1 Related Publications

• An argument for an olfactory thalamus.
• Approaches to the diagnosis of hepatocellular carcinoma.
• Biosynthetic characterization and chemoenzymatic assembly of the cryptophycins. Potent anticancer agents from cyanobionts.
• CRELD1 mutations contribute to the occurrence of cardiac atrioventricular septal defects in Down syndrome.
• Characterization and analysis of early enzymes for petrobactin biosynthesis in Bacillus anthracis.
• Developing tools for engineering hybrid polyketide synthetic pathways.
• Does self-affirmation, cognitive processing, or discovery of meaning explain cancer-related health benefits of expressive writing?
• Endoscopic ultrasound-guided fine-needle aspiration of ascites.
• Examining subtypes of behavior problems among 3-year-old children, Part I: investigating validity of subtypes and biological risk-factors.
• Fewer driver synapses in higher order than in first order thalamic relays.
• Growth-phase dependent differential gene expression in Synechocystis sp. strain PCC 6803 and regulation by a group 2 sigma factor.
• HCV kinetics, quasispecies, and clearance in treated HCV-infected and HCV/HIV-1-coinfected patients with hemophilia.
• Hepatitis C virus coinfection and superinfection.
• Hydroxyquinone O-methylation in mitomycin biosynthesis.
• Interaction of glycolysis and mitochondrial respiration in metabolic oscillations of pancreatic islets.
• Mapping of the functional interconnections between thalamic reticular neurons using photostimulation.
• Metabolic coupling of dehydration and decarboxylation in the curacin A pathway: functional identification of a mechanistically diverse enzyme pair.
• Mice with GFAP-targeted loss of neurofibromin demonstrate increased axonal MET expression with aging.
• Molecular analysis of the role of tyrosine 224 in the active site of Streptomyces coelicolor RppA, a bacterial type III polyketide synthase.
• Neuropsychiatric and behavioral aspects of trisomy 21.
• On socioeconomic gradients in cancer registry data quality.
• Optimal attachment position and linker length promote native-like character of cavitand-based template-assembled synthetic proteins (TASPs).
• Oregon Beach Monitoring Program: Bacterial exceedances in marine and freshwater creeks/outfall samples, October 2002-April 2005.
• Sexual and drug risk behaviors among women who have sex with women.
• Substrate profile analysis and ACP-mediated acyl transfer in Streptomyces coelicolor Type III polyketide synthases.
• Synthetic zeolites and other microporous oxide molecular sieves.
• T cells recognize PD(N/T)R motif common in a variable number of tandem repeat and degenerate repeat sequences of MUC1.
• The National Down Syndrome Project: design and implementation.
• The central role of entitativity in stereotypes of social categories and task groups.
• The prevalence and significance of occult hepatitis B virus in a prospective cohort of HIV-infected patients.