Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis.

Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis. Research Abstract Details 

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Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis. Abstract Text:

Donglin Liu,J Michael Brockman,Brinda Dass,Lucie N Hutchins,Priyam Singh,John R McCarrey,Clinton C MacDonald,Joel H Graber,

Gene expression and processing during mouse male germ cell maturation (spermatogenesis) is highly specialized. Previous reports have suggested that there is a high incidence of alternative 3'-processing in male germ cell mRNAs, including reduced usage of the canonical polyadenylation signal, AAUAAA. We used EST libraries generated from mouse testicular cells to identify 3'-processing sites used at various stages of spermatogenesis (spermatogonia, spermatocytes and round spermatids) and testicular somatic Sertoli cells. We assessed differences in 3'-processing characteristics in the testicular samples, compared to control sets of widely used 3'-processing sites. Using a new method for comparison of degenerate regulatory elements between sequence samples, we identified significant changes in the use of putative 3'-processing regulatory sequence elements in all spermatogenic cell types. In addition, we observed a trend towards truncated 3'-untranslated regions (3'-UTRs), with the most significant differences apparent in round spermatids. In contrast, Sertoli cells displayed a much smaller trend towards 3'-UTR truncation and no significant difference in 3'-processing regulatory sequences. Finally, we identified a number of genes encoding mRNAs that were specifically subject to alternative 3'-processing during meiosis and postmeiotic development. Our results highlight developmental differences in polyadenylation site choice and in the elements that likely control them during spermatogenesis.

Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis. Publishing Authors By Initials

D Liu,JM Brockman,B Dass,LN Hutchins,P Singh,JR McCarrey,CC MacDonald,JH Graber,

For similar congenital, hereditary, and neonatal diseases and abnormalities: genetic diseases, inborn: metabolism, inborn errors: lipid metabolism, inborn errors: hypolipoproteinemias: hypobetalipoproteinemias: abetalipoproteinemia research abstracts see: congenital, hereditary, and neonatal diseases and abnormalities: genetic diseases, inborn: metabolism, inborn errors: lipid metabolism, inborn errors: hypolipoproteinemias: hypobetalipoproteinemias: abetalipoproteinemia research

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Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nucleic acids research

VOLUME: 35

Page Numbers: 234-46

Journal Abbreviation: Nucleic Acids Res.

ISSN: 1362-4962

DAY: 8

MONTH: 12

YEAR: 2006

Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 411011

Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis. Keywords Mesh Terms:

KEYWORDS: mRNA Cleavage and Polyadenylation Factor

MESH TERMS: metabolism

Chemical & Substance for Abstract: Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis. Information

Substance Name: mRNA Cleavage and Polyadenylation Factor

Registry Number: 0

Grant and Affiliation Information for Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis.

AFFILIATION: The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA.

Country: England

AGENCY: United States NICHD

GRANT: HD037102

ACRONYM: HD

MEDLINETA: Nucleic Acids Res

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