System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate.

System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate. Abstract Text:

Deborah U Frank,Sarah A Elliott,Eon Joo Park,Jennetta Hammond,Yukio Saijoh,Anne M Moon,

We targeted the reverse tetracycline controlled transactivator (rtTA) to the Foxa2 locus (Foxa2(ITA)) to generate a system for regulating Cre-recombinase activity within Foxa2 expression domains, including the endoderm, notochord, and floor plate of early mouse embryos. The use of an internal ribosomal entry site to obtain rtTA expression preserves Foxa2 function of the targeted allele. Cre activity with this system reflects the level of endogenous Foxa2 activity and is also tightly controlled by doxycycline. The location of Cre activity within the broader Foxa2 expression domain can be restricted by altering the timing of doxycycline administration. Isolated floor plate expression can be obtained in this manner. This system will provide a useful tool for manipulating gene expression in endoderm, notochord, and floor plate, all of which are tissues with important structural and patterning functions during embryogenesis.

System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate. Publishing Authors By Initials

DU Frank,SA Elliott,EJ Park,J Hammond,Y Saijoh,AM Moon,

For similar genetic structures: genome: genome components: genes: transgenes research abstracts see: genetic structures: genome: genome components: genes: transgenes research

PUBMED ID PMID:

MEDLINE DATE:

System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Developmental dynamics : an official publication o

VOLUME: 236

Page Numbers: 1085-92

Journal Abbreviation:

ISSN: 1058-8388

DAY: 3

MONTH: Apr

YEAR: 2007

System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9201927

System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate. Keywords Mesh Terms:

KEYWORDS: Transgenes

MESH TERMS: metabolism

Chemical & Substance for Abstract: System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate. Information

Substance Name: Integrases

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate.

AFFILIATION: Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah, USA.

Country: United States

AGENCY: United States NICHD

GRANT: R01HD044157-01

ACRONYM: HD

MEDLINETA: Dev Dyn

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate Related Publications

• (Ta/Si) multilayer as a wide-bandpass monochromator material.
• A peroxisome proliferator-activated receptor gamma antagonist induces vimentin cleavage and inhibits invasion in high-grade hepatocellular carcinoma.
• Antisense targeting of TGF-beta1 augments BMP-induced upregulation of osteopontin, type I collagen and Cbfa1 in human Saos-2 cells.
• Aqueous extract of Magnolia officinalis mediates proliferative capacity, p21WAF1 expression and TNF-alpha-induced NF-kappaB activity in human urinary bladder cancer 5637 cells; involvement of p38 MAP kinase.
• Common genetic variation within the low-density lipoprotein receptor-related protein 6 and late-onset Alzheimer's disease.
• Determination of eperisone in human plasma by liquid chromatography-ESI-tandem mass spectrometry.
• Estimation of potential health gains from reducing multiple risk factors of stroke in Korea.
• How home care and hospice nurses can assist adolescents when a parent is seriously ill: adolescents with seriously ill family members.
• Hysteroscopic resection of the vaginal septum in uterus didelphys with obstructed hemivagina: a case report.
• Keeping up to date.
• Microcalcification detection in 3-d breast ultrasound.
• Mineralization of phenol by Ce(IV)-mediated electrochemical oxidation in methanesulphonic acid medium: a preliminary study.
• Neuroprotective activity of triterpenoid saponins from Platycodi radix against glutamate-induced toxicity in primary cultured rat cortical cells.
• On-line analysis of a continuous-flow ozonolysis reaction using Raman spectroscopy.
• Performance measurement of the microPET focus 120 scanner.
• Pomiferin, histone deacetylase inhibitor isolated from the fruits of Maclura pomifera.
• Relations between age, weight, refractive error and eye shape by computerized tomography in children.
• Simple and highly efficient synthesis of 3'-deoxy-3'-[18F]fluorothymidine using nucleophilic fluorination catalyzed by protic solvent.
• Stereoselective [2,3]-sigmatropic rearrangements of unstabilized nitrogen ylides.
• System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate.
• The role of PET/CT for evaluating breast cancer.
• Type I collagen-induced pro-MMP-2 activation is differentially regulated by H-Ras and N-Ras in human breast epithelial cells.
• WNTS and WNT receptors as therapeutic tools and targets in human disease processes.
• [A nationwide survey on current conditions of school health education]
• [Anger, cardiovascular health and depression in middle-aged Korean men: the mediating effect of social support]
• [Expression of caveolin in hepatocellular carcinoma: association with unpaired artery formation and radiologic findings]
• [Health perception, body image, sexual function and depression in menopausal women according to menopausal stages]
• [Renal dysfunction after spontaneous bacterial peritonitis in cirrhosis: incidence and risk factors]
• [Surgical treatment of sclerosing hepatocellular carcinoma]
• [Usefulness of serum alpha-fetoprotein (AFP) as a marker for hepatocellular carcinoma (HCC) in hepatitis C virus related cirrhosis: analysis of the factors influencing AFP elevation without hcc development]