Synthetic sandwich culture of 3D hepatocyte monolayer.

Synthetic sandwich culture of 3D hepatocyte monolayer. Research Abstract Details 

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Synthetic sandwich culture of 3D hepatocyte monolayer. Abstract Text:

Yanan Du,Rongbin Han,Feng Wen,Susanne Ng San San,Lei Xia,Thorsten Wohland,Hwa Liang Leo,Hanry Yu,Yanan Du,Rongbin Han,Feng Wen,Susanne Ng San San,Lei Xia,Thorsten Wohland,Hwa Liang Leo,Hanry Yu,

The sandwich culture of hepatocytes, between double layers of extra-cellular matrix (ECM), is a well-established in vitro model for re-establishing hepatic polarity and maintaining differentiated functions. Applications of the ECM-based sandwich culture are limited by the mass transfer barriers induced by the top gelled ECM layer, complex molecular composition of ECM with batch-to-batch variation and uncontrollable coating of the ECM double layers. We have addressed these limitations of the ECM-based sandwich culture by developing an 'ECM-free' synthetic sandwich culture, which is constructed by sandwiching a 3D hepatocyte monolayer between a glycine-arginine-glycine-aspatic acid-serine (GRGDS)-modified polyethylene terephthalate (PET) track-etched membrane (top support) and a galactosylated PET film (bottom substratum). The bioactive top support and bottom substratum in the synthetic sandwich culture substituted for the functionalities of the ECM in the ECM-based sandwich culture with further improvement in mass transfer and optimal material properties. The 3D hepatocyte monolayer in the synthetic sandwich culture exhibited a similar process of hepatic polarity formation, better cell-cell interaction and improved differentiated functions over 14-day culture compared to the hepatocytes in collagen sandwich culture. The novel 3D hepatocyte monolayer sandwich culture using bioactive synthetic materials may readily replace the ECM-based sandwich culture for liver tissue engineering applications, such as drug metabolism/toxicity testing and hepatocyte-based bioreactors.

Synthetic sandwich culture of 3D hepatocyte monolayer. Publishing Authors By Initials

Y Du,R Han,F Wen,S Ng San San,L Xia,T Wohland,HL Leo,H Yu,Y Du,R Han,F Wen,S Ng San San,L Xia,T Wohland,HL Leo,H Yu,

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Synthetic sandwich culture of 3D hepatocyte monolayer. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biomaterials

VOLUME: 29

Page Numbers: 290-301

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ISSN: 0142-9612

DAY: 26

MONTH: 10

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 8100316

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AFFILIATION: Institute of Bioengineering and Nanotechnology, A*STAR, Singapore 138669, Singapore; Graduate Programme in Bioengineering, Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 117597, Singapore.

Country: England

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MEDLINETA: Biomaterials

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