Synthetic glycogels for affinity electrophoresis: a facile and efficient method for investigating sugar-lectin interaction.

Synthetic glycogels for affinity electrophoresis: a facile and efficient method for investigating sugar-lectin interaction. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Synthetic glycogels for affinity electrophoresis: a facile and efficient method for investigating sugar-lectin interaction. Abstract Text:

M Ohmae,S Suzuki,S Tokura,N Nishi,S I Nishimura,

A novel and efficient method for analyzing sugar-lectin interaction using affinity electrophoresis (AEP) is described. Polyacrylamide gels covalently conjugated with 2-acetamido-2-deoxy-D-glucopyranose (GlcNAc) residues were successfully prepared by radical copolymerization of highly reactive 3-(N-acryloylamino)propyl 2-acetamido-2-deoxy-beta-D-glucopyranoside with acrylamide in the presence of N,N'-methylenebisacrylamide (BIS). When the glycogels carrying various densities of GlcNAc branches were employed for polyacrylamide gel electrophoresis (PAGE) of lectins, the mobilities of wheat germ agglutinin (WGA) were specifically reduced by increasing the concentrations of the GlcNAc residues in gels, although concanavalin A (Con A) showed no significant change in the mobility. It was also demonstrated that the association constant of WGA with immobilized GlcNAc residue can be determined by combined use of this stable glycogel and an automated gel-scanning system associated with fluorometric spectroscopy. The association constant of WGA with the GlcNAc moiety was estimated to be 1.24 x 10(4) M-1.

Synthetic glycogels for affinity electrophoresis: a facile and efficient method for investigating sugar-lectin interaction. Publishing Authors By Initials

M Ohmae,S Suzuki,S Tokura,N Nishi,SI Nishimura,

For similar investigative techniques: chemistry, analytical: spectrum analysis: magnetic resonance spectroscopy research abstracts see: investigative techniques: chemistry, analytical: spectrum analysis: magnetic resonance spectroscopy research

PUBMED ID PMID:

MEDLINE DATE:

Synthetic glycogels for affinity electrophoresis: a facile and efficient method for investigating sugar-lectin interaction. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 119

Page Numbers: 367-71

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1996

Synthetic glycogels for affinity electrophoresis: a facile and efficient method for investigating sugar-lectin interaction. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Synthetic glycogels for affinity electrophoresis: a facile and efficient method for investigating sugar-lectin interaction. Keywords Mesh Terms:

KEYWORDS: Magnetic Resonance Spectroscopy

MESH TERMS: metabolism

Chemical & Substance for Abstract: Synthetic glycogels for affinity electrophoresis: a facile and efficient method for investigating sugar-lectin interaction. Information

Substance Name: Lectins

Registry Number: 0

Grant and Affiliation Information for Synthetic glycogels for affinity electrophoresis: a facile and efficient method for investigating sugar-lectin interaction.

AFFILIATION: Division of Bio-Science, Hokkaido University, Sapporo.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Synthetic glycogels for affinity electrophoresis: a facile and efficient method for investigating sugar-lectin interaction Related Publications

• A New Phenolic Glucoside, Curculigoside from Rhizomes of Curculigo orchioides.
• Action of ascorbic acid on a myosin molecule derived from carp.
• Alginate hydrogel linked with synthetic oligopeptide derived from BMP-2 allows ectopic osteoinduction in vivo.
• Analysis of N-glycan in serum glycoproteins from db/db mice and humans with type 2 diabetes.
• Arteriovenous malformation of the gallbladder: CT and angiographic findings.
• Combination tumor immunotherapy with radiotherapy and Th1 cell therapy against murine lung carcinoma.
• Continence care for older people.
• Dynamic memorization characteristics in neural networks with different neuronal dynamics.
• Evolution of life-history traits collapses competitive coexistence.
• Fluid-attenuated inversion-recovery SSFP imaging.
• Hemodynamics of ovarian veins: MR angiography in women with uterine leiomyomata.
• High glucose up-regulates lipopolysaccharide-stimulated inflammatory cytokine production via c-jun N-terminal kinase in the monocytic cell line THP-1.
• High peak inspiratory flow can aggravate ventilator-induced lung injury in rabbits.
• Influence of human CD8 on antigen recognition by T-cell receptor-transduced cells.
• Knockout of glutelin genes which form a tandem array with a high level of homology in rice by gamma irradiation.
• Management of urethral hemangiomas associated with Klippel-Trenaunay-Weber syndrome by endoscopic sclerotherapy.
• Multitensor tractography enables better depiction of motor pathways: initial clinical experience using diffusion-weighted MR imaging with standard b-value.
• PET measurement of FK506 concentration in a monkey model of stroke.
• Pharmacokinetics of telithromycin using bronchoscopic microsampling after single and multiple oral doses.
• Pitfall of percutaneous cardiopulmonary support for acute massive pulmonary embolism.
• Polarization of Healpha radiation due to anisotropy of fast-electron transport in ultraintense-laser-produced plasmas.
• Randomized placebo-controlled trial of interferon alpha-2b plus ribavirin with and without lactoferrin for chronic hepatitis C.
• Resilience, family adversity and well-being among Hawaiian and non-Hawaiian adolescents.
• Short term prediction of Ht value using BPNN during LCAP.
• Some characteristics of fluoride-induced cell death in rat thymocytes: cytotoxicity of sodium fluoride.
• Sternum fracture and haemorrhage after cardiopulmonary resuscitation.
• T cell immunotherapy.
• Three-dimensional bone-implant integration profiling using micro-computed tomography.
• Tumour necrosis factor alpha production by polymorphonuclear neutrophils treated with mouse anti-human CD19 monoclonal antibody.
• [Recent progress in paroxysmal nocturnal hemoglobinuria]