Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes.

Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes. Research Abstract Details 

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Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes. Abstract Text:

Christine B Karim,Zhiwen Zhang,David D Thomas,Christine B Karim,Zhiwen Zhang,David D Thomas,

A procedure is described for the synthetic incorporation into membrane proteins of the non-natural amino acid TOAC (2,2,6,6-tetramethyl-piperidine-1-oxyl-4-amino-4-carboxylic acid), which is coupled rigidly to the alpha-carbon, providing direct detection of peptide backbone dynamics by electron paramagnetic resonance (EPR). Also included is a protocol for the functional reconstitution of the spin-labeled protein in lipid vesicles. This protocol can be completed in 17 d.

Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes. Publishing Authors By Initials

CB Karim,Z Zhang,DD Thomas,CB Karim,Z Zhang,DD Thomas,

For similar spin labels research abstracts see: spin labels research

PUBMED ID PMID:

MEDLINE DATE:

Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nature protocols

VOLUME: 2

Page Numbers: 42-9

Journal Abbreviation:

ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2007

Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101284307

Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes. Keywords Mesh Terms:

KEYWORDS: Spin Labels

MESH TERMS: chemical synthesis

Chemical & Substance for Abstract: Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes. Information

Substance Name: 2,2,6,6-tetramethylpiperidine-N-oxide-4-

Registry Number: 15871-57-5

Grant and Affiliation Information for Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes.

AFFILIATION: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA. cbk@ddt.biochem.umn.edu

Country: England

AGENCY: United States NIGMS

GRANT: GM27906

ACRONYM: GM

MEDLINETA: Nat Protoc

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