Synthesis of ribosomal structural proteins by postmitochondrial supernatant from regenerating rat liver.

Synthesis of ribosomal structural proteins by postmitochondrial supernatant from regenerating rat liver. Research Abstract Details 

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Synthesis of ribosomal structural proteins by postmitochondrial supernatant from regenerating rat liver. Abstract Text:

K Tsurgi,K Ogata,

1. When the postmitochondrial supernatant (PM-supernatant) from regenerating rat liver was incubated with [3H]methionine, the incorporation of [3H]methinoine into the N-terminal residues of nascent peptides on ribosomes was observed. This incorporation was sensitive to a low cocentration (2X10(-6) M) of pactamycin. The results suggest that PM-supernatant has low but definite activity for the initiation of nascent protein synthesis. Polysomes and cell sap from regenerating rat liver showed negligible pactamycin-sensitive incorporation of [3H]methionine into N-terminal, residues of nascent peptides. 2. PM-supernatant from regenerating rat liver was incubated with [35S]methionine in the complete reaction mixture. After addition of ribosomal proteins labelled with [3H]methionine in vivo, ribosomal structural proteins were prepared from the incubation mixture by acetic acid extraction, CM-cellulose column chromatography, Sephadex G-200 gel filtration and finally by two-dimensional acrylamide gel electrophoresis. Incorporation was observed in the greater part of ribosomal proteins on the two dimensional gel. From the 35S-to-3H ratios of ribsomal protein fractions during the purification procedures, it appeared that the incorporation of labelled methionine into the ribosomal proteins by PM-supernatant was about 3% of that into the total proteins. When [3H]leucine was used, the values were about 4% in the same cell-free system and 5 to 6% in in vivo labelling. The results indicate that ribosomal proteins are synthesized with high efficiency by PM-supernatant from regenerating rat liver.

Synthesis of ribosomal structural proteins by postmitochondrial supernatant from regenerating rat liver. Publishing Authors By Initials

K Tsurgi,K Ogata,

For similar cells: cellular structures: subcellular fractions research abstracts see: cells: cellular structures: subcellular fractions research

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Synthesis of ribosomal structural proteins by postmitochondrial supernatant from regenerating rat liver. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 79

Page Numbers: 883-93

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1976

Synthesis of ribosomal structural proteins by postmitochondrial supernatant from regenerating rat liver. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Synthesis of ribosomal structural proteins by postmitochondrial supernatant from regenerating rat liver. Keywords Mesh Terms:

KEYWORDS: Subcellular Fractions

MESH TERMS: metabolism

Chemical & Substance for Abstract: Synthesis of ribosomal structural proteins by postmitochondrial supernatant from regenerating rat liver. Information

Substance Name: RNA

Registry Number: 63231-63-0

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Country: JAPAN

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MEDLINETA: J Biochem

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