Synthesis of recombinant human single-chain urokinase-type plasminogen activator variants resistant to plasmin and thrombin.

Synthesis of recombinant human single-chain urokinase-type plasminogen activator variants resistant to plasmin and thrombin. Research Abstract Details 

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Synthesis of recombinant human single-chain urokinase-type plasminogen activator variants resistant to plasmin and thrombin. Abstract Text:

T Miyake,Y Hibino,Y Kobayashi,M Yokoyama,K Watabe,M Ohmori,

Single-chain urokinase-type plasminogen activator (scu-PA), a potential therapeutic reagent for thrombosis, is activated in plasma by plasmin. The activated enzyme is further digested by plasmin to generate low-molecular-weight urokinase (LMW-UK), which has no affinity for fibrin. To circumvent this dual effect of plasmin, we synthesized in Escherichia coli a variant of scu-PA, which is not converted to LMW-UK on treatment with plasmin. In another variant, the activation cleavage site was modified such that activation by plasmin was slowed down and that inactivation by thrombin was greatly diminished. The combination of these variants may be applicable as an effective thrombolytic reagent for clinical use.

Synthesis of recombinant human single-chain urokinase-type plasminogen activator variants resistant to plasmin and thrombin. Publishing Authors By Initials

T Miyake,Y Hibino,Y Kobayashi,M Yokoyama,K Watabe,M Ohmori,

For similar genetic phenomena: variation (genetics) research abstracts see: genetic phenomena: variation (genetics) research

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Synthesis of recombinant human single-chain urokinase-type plasminogen activator variants resistant to plasmin and thrombin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 104

Page Numbers: 643-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1988

Synthesis of recombinant human single-chain urokinase-type plasminogen activator variants resistant to plasmin and thrombin. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Synthesis of recombinant human single-chain urokinase-type plasminogen activator variants resistant to plasmin and thrombin. Keywords Mesh Terms:

KEYWORDS: Variation (Genetics)

MESH TERMS: genetics

Chemical & Substance for Abstract: Synthesis of recombinant human single-chain urokinase-type plasminogen activator variants resistant to plasmin and thrombin. Information

Substance Name: Urinary Plasminogen Activator

Registry Number: EC 3.4.21.73

Grant and Affiliation Information for Synthesis of recombinant human single-chain urokinase-type plasminogen activator variants resistant to plasmin and thrombin.

AFFILIATION: Sagami Chemical Research Center, Kanagawa.

Country: JAPAN

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MEDLINETA: J Biochem

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