Synthesis of nitric oxide in human osteoblasts in response to physiologic stimulation of electrotherapy.

Synthesis of nitric oxide in human osteoblasts in response to physiologic stimulation of electrotherapy. Research Abstract Details 

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Synthesis of nitric oxide in human osteoblasts in response to physiologic stimulation of electrotherapy. Abstract Text:

Ayman Hamed,Paul Kim,Michael Cho,

Electrotherapy for bone healing, remodeling and wound healing may be mediated by modulation of nitric oxide (NO). Using NO-specific fluorophore (DAF-2), we report here that application of non-invasive, physiologic electrical stimulation induces NO synthesis in human osteoblasts, and that such NO generation is comparable to that induced by estrogen treatment. For example, application of a sinusoidal 1 Hz, 2 V/cm (peak to peak) electrical stimulation (ES) increases NO-bound DAF-2 fluorescence intensity by a 2-fold within 60 min exposure by activating nitric oxide synthase (NOS). Increase in the NO level is found to depend critically on the frequency and strength of ES. While the frequency of 1 Hz ES seems optimal, the ES strength >0.5 V/cm is required to induce significant NO increase, however. Nitric oxide synthesis in response to ES is completely prevented by blocking estrogen receptors using a competitive inhibitor, suggesting that NO generation is likely initiated by activation of estrogen receptors at the cell surface. Based on these findings, physiologic stimulation of electrotherapy appears to represent a potential non-invasive, non-genomic, and novel physical technique that could be used to regulate NO-mediated bone density and facilitate bone remodeling without adverse effects associated with hormone therapy.

Synthesis of nitric oxide in human osteoblasts in response to physiologic stimulation of electrotherapy. Publishing Authors By Initials

A Hamed,P Kim,M Cho,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Synthesis of nitric oxide in human osteoblasts in response to physiologic stimulation of electrotherapy. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Annals of biomedical engineering

VOLUME: 34

Page Numbers: 1908-16

Journal Abbreviation:

ISSN: 0090-6964

DAY: 26

MONTH: 10

YEAR: 2006

Synthesis of nitric oxide in human osteoblasts in response to physiologic stimulation of electrotherapy. Information

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LANGUAGE: eng

NlmUniqueID: 361512

Synthesis of nitric oxide in human osteoblasts in response to physiologic stimulation of electrotherapy. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Synthesis of nitric oxide in human osteoblasts in response to physiologic stimulation of electrotherapy. Information

Substance Name: Fluorescein

Registry Number: 2321-07-5

Grant and Affiliation Information for Synthesis of nitric oxide in human osteoblasts in response to physiologic stimulation of electrotherapy.

AFFILIATION: Department of Bioengineering, University of Illinois at Chicago, 851 S. Morgan St. (M/C 063), Chicago, IL, 60607, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM060741

ACRONYM: GM

MEDLINETA: Ann Biomed Eng

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