Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene.

Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene. Abstract Text:

K D Preuss,E Krauhs,K H Scheit,

A synthetic DNA, carrying the coding sequence for seminalplasmin (SAP), the major basic protein of bull semen, was cloned into the C-terminal part of a shortened, mutated fragment of the lacZ gene (lacZ-MF) of vector pLZPWB1. As a result of the mutation, all methionine as well as cysteine residues are replaced by other amino-acid residues. In the fusion gene lacZ-MF-SAP of the resulting construct pSAP4 the two proteins are linked through a methionine residue. Expression of pSAP4 in E. coli W3110 in the presence of the inducer isopropylthiogalactoside (IPTG) led to production of fusion protein with a yield of approximately 50% of the total proteins synthesized. All SAP-immunoreactive fusion protein was found within the insoluble protein fraction and represented 40% of total proteins produced during expression. The fusion protein was subjected to cyanogen bromide cleavage. The overall yield of crude SAP with a purity of 80% was 10 mg/l of culture. The crude SAP was further purified by calmodulin-Sepharose affinity absorption. Characterisation by protein chemical analysis indicated the identity of recombinant SAP with authentic SAP purified from bull semen.

Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene. Publishing Authors By Initials

KD Preuss,E Krauhs,KH Scheit,

For similar proteins: seminal plasma proteins: seminal vesicle secretory proteins research abstracts see: proteins: seminal plasma proteins: seminal vesicle secretory proteins research

PUBMED ID PMID:

MEDLINE DATE:

Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biological chemistry Hoppe-Seyler

VOLUME: 371

Page Numbers: 215-22

Journal Abbreviation: Biol. Chem. Hoppe-Seyler

ISSN: 0177-3593

DAY: 19

MONTH: Mar

YEAR: 1990

Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8503054

Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene. Keywords Mesh Terms:

KEYWORDS: Seminal Vesicle Secretory Proteins

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene. Information

Substance Name: Seminal Vesicle Secretory Proteins

Registry Number: 0

Grant and Affiliation Information for Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene.

AFFILIATION: Max-Planck-Institut für Biophysikalische Chemie, Abt. Molekulare Biologie, Göttingen.

Country: GERMANY, WEST

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biol Chem Hoppe Seyler

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene Related Publications

• Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen.
• Characterization of the gene for seminalplasmin, a secretory protein of the bovine seminal vesicle.
• Effect of seminal plasmin on rRNA synthesis in Saccharomyces cerevisiae.
• Functional properties of peptides derived from seminalplasmin: binding to monospecific anti-seminalplasmin immunoglobulins G and calmodulin.
• Immunohistochemistry of secretory proteins in the bull seminal vesicle.
• Immunological identification of seminalplasmin in tissue extracts of sex glands of bull.
• Promoter analysis of the bovine gene for seminalplasmin.
• Purification and spectral characterization of seminalplasmin, an antimicrobial protein from bull semen.
• Seminalplasmin, the major basic protein of bull seminal plasma, is a secretory protein of the seminal vesicles.
• Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene.
• [A quantitative ELISA for the measurement of class-specific anti-spermatozoa antibodies]